清开灵注射液对心肌缺血-再灌注损伤大鼠凋亡相关蛋白Caspase表达的影响  

Effects of Qingkailing Injection on apoptosis related protein expression of caspase in myocardial ischemia-reperfusion injurty rats

作　　者：赵丽红[1] 赵立勤[2] 于艳华[1] 石卓[3] 

机构地区：[1]白城医学高等专科学校,吉林白城137000 [2]大安市中医院,吉林大安131300 [3]吉林大学白求恩医学院药理学系,吉林长春130021

出　　处：《中国实验诊断学》2012年第7期1171-1173,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省教育厅"十一五"科学技术研究项目(吉教科合字【2008】第439号)

摘　　要：目的探讨清开灵注射液(QKL)对心肌缺血—再灌注损伤后大鼠心肌细胞凋亡相关蛋白Caspase表达的影响及其作用机制。方法实验共设假手术组、模型组、复方丹参注射液组(DS组)及清开灵注射液组(QKL治疗组),每组8只,各组术前连续舌下静脉给药1周,采用结扎大鼠左冠状动脉前降支40min后,松解再灌注120min,制备心肌缺血—再灌注模型,实验结束后HE染色观察心肌细胞形态学变化,免疫组化观察心肌Caspase-3、Caspase-9表达情况。结果 QKL能够下调心肌细胞Caspase-3、Caspase-9表达,从而减少心肌细胞的凋亡。结论 QKL对大鼠心肌缺血-再灌注损伤具有保护作用,其保护作用的机制与下调心肌细胞凋亡相关蛋白Caspase-3、Caspase-9表达有关。Objective To explore the effects of Qingkailing（QKL） on apoptosis in myocardia of rats after ischemic-reperfusion.Methods Rats were divided into sham,model,DanShen and Qingkailing groups at random,8 rats in each,and were treated with medicines by sublingual vein administration respectively last 1 week.All rats were subjected to openning chest then the left anterior desending coronary arteries（LAD） were ligated for 40 min and unclamped for 120 min,at the end of,myocardial pathological changes were observed by light microscope after HE dyeing,and measure the apoptotic cells by TUNEL to observe the expressiones of caspase-3 and caspase-9 by immunohistochemistry.Results QKL downregulated the expressiones of Caspase-3,Caspase-9.Conclusion QKL have protective effects on ischemiareperfusion in rats,which may be related to downregulated the expressiones of Caspase-3,Caspase-9 during ischemia-reperfusion.

关 键 词：清开灵注射液 心肌缺血-再灌注损伤 凋亡 Caspase-3 CASPASE-9 

分 类 号：R285.5[医药卫生—中药学]