ELISA、GICA与MEIA法检测新生儿TORCH-IgM抗体的比较  

The Comparison of ELISA,GICA and MEIA for the Detection of TORCH-IgM Antibody in Newborn

作　　者：邹建话[1] 江凡[1] 罗惠玲[1] 张修发[1] 高水湾[1] 周喜友[1] 张慧莲[1] 

机构地区：[1]深圳市宝安妇幼保健院检验科,广东深圳518133

出　　处：《中国实验诊断学》2012年第7期1224-1226,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的比较ELISA、GICA与MEIA法对新生儿TORCH-IgM抗体的检测效果。方法选择120例疑似TORCH感染的新生儿作为研究对象,分别采用ELISA、GICA与MEIA三种方法检测血清TORCH-IgM抗体,比较三种方法对新生儿TORCH-IgM抗体检测的阳性率、符合率、灵敏度以及特异性。结果以MEIA法为金标准,ELISA法和GICA法检测TORCH-IgM抗体的灵敏度和特异性比较,ELISA法检测中的RV-IgM、HSV-IgM以及CMV-IgM检测的灵敏度均高于GICA法(P<0.01),两组的TOX-IgM检测比较无显著性差异(P>0.05);ELISA法检测中的CMV-IgM检测的特异性高于GICA法(P<0.01);ELISA法和GICA法检测TORCH-IgM抗体阳性预测值和阴性预测值比较,ELISA法中CMV-IgM检测的阳性预测值和阴性预测值均高于GICA法(P<0.05),尤其是阴性预测值(P<0.01);与MEIA法检测总符合率均在90%以上。结论 ELISA法检测新生儿TORCH-IgM抗体,具有特异性强、敏感性高、可信度高、简单易行等优点,是一种比较可靠的新生儿TORCH感染筛查的检测方法。Objective To compare the detection effect of ELISA,GICA and MEIA on TORCH-IgM antibody in newborn.Methods 120 newborn with suspected TORCH infection were chose as study object.Their serum TORCH-IgM antibody were detected with ELISA,GICA and MEIA,respectively.The positive rate,coincidence rate,sensitivity and specificity of three methods were comparedResults To the gold standard for MEIA method,RV-IgM,HSV-IgM CMV and the sensitivity of testing-IgM of ELISA were higher than GICA method（P0.01） when ELISA and GICA method to detect the TORCH-IgM antibody specificity and sensitivity comparison,and there was no significant difference on TOX-IgM（P0.05）.Take MEIA as golden standard,the CMV-IgM coincidence rate of ELISA is higher than that of GICA（P0.05） when ELISA and GICA method to detect TORCH-IgM antibody positive and negative prediction is predicted.Sensitivity detection of CMV-IgM,HSV-IgM and CMV-IgM in ELISA are higher than that in GICA（P0.01）,and CMV-IgM specificity detection of ELISA is higher than that of GICA（P0.01）.The positive predictive value and negative predictive value of CMV-IgM detection in ELISA are higher than that in GICA（P0.05）,especially for negative predictive value（P0.01）.And MEIA method to detect the total coincidence rate were above 90%.Conclusion There are high sensitivity and specificity of strong,high reliability,simple,etc,when ELISA test neonatal TORCH-IgM antibody.It is a kind of reliable newborn screening TORCH infection test methods.

关 键 词：TORCH 酶联免疫吸附试验 微粒子酶免疫法 胶体金免疫层析法 

分 类 号：R446.6[医药卫生—诊断学]