浊点萃取法提取贯叶连翘总黄酮的研究  

Research on the extraction of flavone from Hypericum perforatum L.by cloud point extraction

作　　者：刘超 罗晶 马辉 张爱丽[3] 陈惠[3] 

机构地区：[1]西安市中医医院药剂科,西安710001 [2]陕西省食品药品检验所,西安710061 [3]第四军医大学药学院,西安710032

出　　处：《西北药学杂志》2012年第4期296-298,共3页Northwest Pharmaceutical Journal

基　　金：陕西省中医管理局中医药基金项目(编号:zy16)

摘　　要：目的建立浊点萃取提取贯叶连翘中总黄酮的工艺。方法采用浊点萃取法进行总黄酮提取,并用紫外分光光度法测定其含量。用正交实验法确定表面活性剂质量浓度、液固比、离子强度、平衡时间等关键因素对提取率的影响,优化提取工艺。结果采用非离子表面活性剂聚氧乙烯脂肪醇醚Genapol-X 80,质量浓度为50.0g.L-1,液固比为100∶1,NaCl浓度为3.0mol.L-1,在65℃下,萃取40min,达到最高的提取率,相对于传统工艺,提取率大大提高。结论该方法稳定易行,操作简单,绿色环保,避免高温破坏,提取率高。Objective To establish a extraction process of flavone from Hypericum perforatum L. by cloud point extraction（ CPE）. Methods Cloud point extraction was adopted to the extraction of flavone,and UV was used for detection. By an orthogonal experiment, the effect of surfactant concentration, solid-liquid ratio,ionic strength and balance time on the extraction efficiency was studied. Result When extraction was processed under the conditions of 50.0 g·L-1 surfactant concentration, 100：1 solid：liquid ratio, 3.0 mol·L-1 salt concentration for 40 min under 65℃ ,the extraction ratio was the highest. The method is superior to the traditional method. Conclusion CPE is a green, steady, reasonable and feasible extraction method.

关 键 词：贯叶连翘 总黄酮 浊点萃取 

分 类 号：R284[医药卫生—中药学]