氢醌对DNA甲基转移酶表达的影响  

Effects of Hydroquinone on the Expression of DNA Methyltransferase

作　　者：凌晓璇[1,2] 梁海荣[1,2] 黄明元[3,2] 杨慧[1,2] 唐焕文[3,2] 

机构地区：[1]广东医学院公共卫生学院实验中心,广东东莞523808 [2]广东医学院环境与健康研究所 [3]广东医学院公共卫生学院劳动卫生与环境卫生学教研室

出　　处：《实用预防医学》2012年第7期961-963,共3页Practical Preventive Medicine

基　　金：国家自然科学基金(30972500);广东省自然科学基金(7301507);东莞市科技计划项目(201010815206)

摘　　要：目的探索氢醌(hydroquinone,HQ)致DNA整体低甲基化的分子机制。方法以磷酸盐缓冲液(PBS)溶解HQ,以PBS处理组为对照组,分别以2.5、5.0、10.0和20.0μmo/lL HQ染毒TK6细胞为处理组。应用实时荧光定量-聚合酶链反应检测DNA甲基转移酶DNMT1、DNMT3a和DNMT3b的表达水平。结果与对照组相比,DNMT1、DNMT3a和DNMT3b的mRNA表达量在各HQ处理组细胞中均下降,其中以20.0μmo/lL组细胞的下降最为明显,分别下降46%(P<0.05)、83%(P<0.05)和48%(P<0.05),且DNMT1和DNMT3a的表达量随着HQ剂量的增加而下降。结论 HQ致DNA整体低甲基化下调机制可能与DNMT1、DNMT3a和DNMT3b表达异常有关。Objective To explore the molecular mechanisms of global DNA hypomethylation induced by hydroquinone（HQ）.Methods TK6 cells were exposed to 2.5,5.0,10.0 and 20.0 μmol/L HQ prepared by PBS buffer,and TK6 cells treated with PBS served as the control.Expressions of DNA methyltransferase（DNMT）,DNMT1,DNMT3a and DNMT3b,were tested by real-time fluorescence quantitive PCR.Results Expressions of DNMT1,DNMT3a and DNMT3b mRNA in the HQ-treated cells were severely inhibited as compared to those in the control cells,with the most remarkable inhibition at 20 μmol/L,showing a decrease by 46%,83% and 48% respectively（all P0.05）.The expressions of DNMT1 and DNMT3a showed more decrease with the increase of HQ dose.Conclusions Global DNA hypomethylation induced by hydroquinone may be associated with the deregulated expressions of DNMT1,DNMT3a and DNMT3b.

关 键 词：氢醌 DNA甲基转移酶 基因表达 

分 类 号：R994.6[医药卫生—毒理学]