不同蛋白源饲粮下断奶仔猪小肠中增食欲素受体2的表达特征  

Effect of Different Weaning Diets on Expression Pattern of Hypocreins Receptor 2 in Intestine for Weanling Piglets

作　　者：孙云子[1] 陈代文[2] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001 [2]四川农业大学动物营养研究所,四川雅安625014

出　　处：《畜牧与饲料科学》2012年第7期1-3,共3页Animal Husbandry and Feed Science

基　　金：四川省教育厅重点项目;贵州师范大学博士启动基金项目

摘　　要：为探讨不同蛋白源饲粮对断奶仔猪小肠中增食欲素受体2(HCRTR2)的表达特征,研究采用实时荧光定量PCR方法检测HCRTR2的mRNA在断奶后分别饲喂动物蛋白替代30%总蛋白饲粮(处理1)及全植物性蛋白饲粮(处理2)0、3、7、14日龄大约克猪空肠中的表达特征。结果表明,不同蛋白源饲粮对HCRTR2基因mRNA的表达均有影响;与断奶0日龄相比,在处理1中,断奶3、7和14日龄时,HCRTR2的表达分别上升了4.30、6.60和4.12倍;处理2中,断奶3、7、14日龄HCRTR2基因mRNA的表达量分别为断奶0日龄时的0.71、1.44和4.50倍。结论:不同蛋白源饲粮对HCRTR2基因mRNA的表达均有影响。To investigate the impact of different weanling diets on expression pattern of Hypocreins Receptor 2 （HCRTR2） in intestine for weanlingpiglets,real-time quantitative reverse transcription PCR （RT-qPCR） was carried out to quantify HCRTR2 expression at age of 0,3,7 and 14 days in weanling piglets fed with the diets 30% milk protein substituted the whole protein （treatment 1 ） and the vegetable protein （treatment 2）.RT-qPCR result showed that HCRTR2 expressed in postnatal intestine of piglets during weanling. In the treatment 1,the expression level increased on day 3,7,14 compared with day 0 with 4.30,6.60 and 4.12 times higher respectively and expression on day 7 being highest.In the treatment 2,the expression level increased 1.44 and 4.50 times on day 7,14 while it decreased 0.71 times on day 3 compared with day 0. In conclusion,the different protein diets affect the expression pattern of Hypocreins Receptor 2 in intestine for weanling piglet.

关 键 词：增食欲素受体2 空肠 表达特征 断奶仔猪 

分 类 号：S828.1[农业科学—畜牧学]