水稻变异后代苗期耐低钾基因型的筛选  

Screening and Evaluation of Low-Potassium Tolerant Genotypes from Rice Offsprings at Seedling Stage

作　　者：库文珍[1] 萧浪涛[2] 彭克勤[2] 

机构地区：[1]湖南城市学院化学与环境工程学院,湖南益阳413000 [2]湖南农业大学植物激素与生长发育湖南省重点实验室,长沙410128

出　　处：《湖南城市学院学报（自然科学版）》2012年第2期57-60,共4页Journal of Hunan City University:Natural Science

摘　　要：对30个来自富钾植物商陆DNA导入受体水稻品种粳85-5得到的性状稳定的变异后代材料在低钾营养液中进行培养,测定其三叶期幼苗K+吸收和H+释放速率.结果表明,在低钾溶液中,不同基因型水稻的K+吸收和H+释放速率差异显著,两者之间呈极显著正相关,变异后代的K+吸收和H+释放速率均比其受体的高.结合考察30个水稻基因型分蘖期的株高、苗干重、根干重,对其进行耐低钾能力评价,这些基因型水稻被分为1级(高)、2级(中)、3级(非)耐低钾基因型3类.DNA from K-rich plants Phytolaoca esculenta Van Houtt were introduced into a variety of Oryza Sativa japonica 85-5. From their offspring 30 plants were chosen as experimental materials for screening low-K tolerant rice genotypes at seedling stages by solution culture. K＋ influx and H^＋ efflux of thirty-two rice varieties materials had been measured at low K level. The result showed that K^＋ influx rate and H＋ efflux rate of low-K tolerant rice genotypes are higher than those of their recipient. There was positive correlation between K^＋ influx rate and H^＋ efflux rate of different rice genotypes. Meanwhile, the low-K tolerant rice genotypes were further screening identified by measuring their height, dry weight of shoots and roots of the 30 rice varieties at tillering stage under normal and low K levels. The 30 rice varieties were differentiated into high, middle and low grades of gentype tolerant to K defieieney.

关 键 词：水稻 低钾胁迫 筛选 

分 类 号：Q945.78[生物学—植物学]