SSR分子标记技术在瓠瓜种子纯度快速鉴定中的应用  

A rapid and precise SSR-based procedure for bottle gourd seed quality survey

作　　者：鲁忠富[1] 徐沛[1] 吴晓花[1] 汪宝根[1] 王莎[1] 刘永华[1] 李国景[1] 

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2012年第4期578-581,共4页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅重大优先主题项目(2008C120031)

摘　　要：当前生产上应用的瓠瓜品种众多,且商业品种间的遗传相似性愈来愈高,种子质量纠纷时有发生。为提供一种快速、准确、可靠的瓠瓜种子纯度检测方法,本研究以44份瓠瓜种质为试材,通过高通量测序自主设计开发适用于瓠瓜研究的SSR引物,从中筛选出8对诊断性引物,在此基础上建立基于SSR分子标记技术的瓠瓜种子纯度快速检测方法。根据本方法建立的瓠瓜主栽品种标准DNA指纹可高效鉴别瓠瓜种子真伪及纯度,满足瓠瓜种子产业化健康发展的需要。Current application of large number of commercial bottle gourd cultivars with very high genetic similarity has caused many disputes on seed quality. To provide a rapid, precise and reliable method for bottle gourd seed puri- ty assay, we developed a set of SSR markers based on DNA sequences obtained from high-throughput sequencing. Eight of these markers were defined as diagnostic markers by genotyping a collection of 44 bottle gourd genotypes. Based on this, a rapid and precise SSR-based procedure as well as standard DNA fingerprints for bottle gourd seed quality survey was established, which will be beneficial for the industrialization of bottle gourd seeds.

关 键 词：瓠瓜 SSR 纯度鉴定 遗传指纹图谱 

分 类 号：S652.9[农业科学—果树学]