生防菌株MT-06对黄瓜白粉病的防治及其定殖力测定  

Evaluation of biological control strain MT-06 for managing cucumber powdery mildew by pot tests and its colonization measurement on cucumber

作　　者：彭志荣[1,2] 毛雪琴[2] 姜华[2] 柴荣耀[2] 张震[2] 王艳丽[2] 王教瑜[2] 邱海萍[2] 孙国昌[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省植物有害生物防控重点实验室一省部共建国家重点实验室培育基地,浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2012年第4期624-629,共6页Acta Agriculturae Zhejiangensis

基　　金：浙江省农业科学院国际合作项目(2009)

摘　　要：不同MT-06发酵液、孢子液和菌液防治黄瓜白粉病的盆栽试验研究发现:不同浓度梯度MT-06制剂对黄瓜白粉病都有抑制作用。MT-06菌液10倍稀释液处理3 d后的保护效果和治疗效果分别达到87.86%和84.89%,与对照三唑酮的处理效果相当,但处理11 d后MT-06菌液10倍稀释液的治疗效果显著高于三唑酮处理(P<0.05),其他处理防治效果都未能达到三唑酮的防治效果。MT-06菌液和孢子液表现防效较MT-06发酵液持久。MT-06定殖试验表明:叶柄上的定殖率仅1×105个.mL-1MT-06孢子液为85%,其余孢子液及菌液处理定殖率均为100%;MT-06在叶片上定殖率较低,MT-06菌液10倍稀释液的定殖率最高为40%。分析认为MT-06防效的保持性较好可能与其能定殖于植物上有关。The evaluation was carried out to determine the ability of Talaromyces flavus, MT-06-based products in management of cucumber powdery mildew, caused by Sphaerotheca fuliginea. The resuhs showed that the effective- ness of strain MT-06 was dependent on its concentration in pot tests. In particular, lO-fold dilution of mycelium mixed liquid have the best effect, with the preventive effects of 87.86% and the curative effects of 84. 89% compared to the control after spraying 11 days, while the effect of the other treatments of strain MT-06 are similar to the chemical fungicide triadimefon. The effect of fermented liquid decreased with the prolonging of time; however, the effect of myeelium mixed liquid and spore suspension kept stable for a long time. The colonization test of strain MT- 06 on cucumber plants showed that petiole colonization rate is higher than that of the leaves. The petiole colonization rate of mycelium mixed liquid and spore suspension was 100%, except when sprayed the spore suspension at the co centration of 1× 105 mL-1 was 85%, while the highest colonization rate on cucumber leaves was 40%. Therefore, the biocontrol efficiency of strain MT-06 may be partially attributed to the ability of colonization on cucumber plants. Overall, these results showed that strain MT-06 played an important role in managing powdery mildew on cucumber plants.

关 键 词：黄蓝状菌 黄瓜白粉病 MT-06 生防 

分 类 号：S482.39[农业科学—农药学]