月见草油抑制草酸钙结晶形成的实验研究  

Experimental study of the inhibitory effect of γ-linolenic acid on calcium oxalate crystalization in rats

作　　者：张海滨[1] 石玮[1] 岳中瑾[1] 

机构地区：[1]兰州大学第二医院泌尿外科,甘肃兰州730030

出　　处：《现代泌尿外科杂志》2012年第4期380-383,共4页Journal of Modern Urology

摘　　要：目的了解月见草油在草酸钙结石形成中的作用,为临床治疗提供新的方法与思路。方法雄性SD大鼠60只,随机分为4组,各组15只。C组和D组以月见草油(含γ-亚麻酸9.2%)或葵花籽油(含亚油酸70%)10g/kg灌胃4周后,用诱石剂1%乙二醇(EG)加2%氯化氨喂饮,同时继续以月见草油或葵花籽油灌胃4周,8周后检测各组大鼠肾功能、24h血尿生化指标和肾草酸钙结晶情况;仅饲普通饲料(A组,空白组)和普通饲料加1%乙二醇(EG)加2%氯化氨喂饮(B组,成石组)大鼠作为对照。结果月见草油组肾组织水肿较轻,肾内草酸钙结晶数及肾成石率低于成石组(P<0.05),尿枸橼酸较成石组高(P<0.01),24h尿钙、尿草酸排泄均低于成石组(P<0.01),血尿素氮(P<0.01)、血肌酐(P<0.05)低于成石组。结论γ-亚麻酸能有效改善肾功能,减少尿钙及草酸的排泄,抑制实验鼠肾草酸钙结晶形成,在尿石症防治方面可能有一定应用价值。Objective To compare the role of γ-linolenic acid（γ-LNA） in the prevention of stone-forming with that of linoleic acid（LNA）.Methods 60 male adult SD rats were divided into 4 groups,group A（normal contro1）,group B（stone forming）,group C（evening primrose oil,9.2% γ-LNA）,and group D（sunflower seed oil,70% LN）.Rats in group C were fed with evening primrose oil and rats in group D with sunflower seed oil for 4 weeks.Renal stone formation was induced by 1% ethylene glycol（EG） plus 2% muriate.Meanwhile,gavage was continued with evening primrose oil and sunflower seeds oil.After 8 weeks,all rats were sacrificed and the renal function,24 h blood and urine biochemical indexes,renal calcium oxalate crystallization and urinary oxalate were detected.Results The parenchymal edema in group C were milder compared with that in group B.Calcium oxalate crystallization,urinary calcium excretion（P〈0.01）,urinary oxalate（P〈0.01）,blood urea nitrogen（P〈0.01） and creatinine（P〈0.05） were lower in group C than in group B,but urinary citrate was higher in group C than in group B（P〈0.01）.Conclusions It is suggested that γ-linolenic acid can protect and improve renal function,decrease urinary calcium and urinary oxalate excretion,and prevent calcium oxalate crystal formation.It may be valuable for the prevention and treatment of urolithiasis.

关 键 词：泌尿系结石 草酸钙 Γ-亚麻酸 月见草油 草酸钙结晶 

分 类 号：R691.4[医药卫生—泌尿科学]