HA纳米微粒对PEG-600低温保护剂反玻璃化结晶的影响  

EFFECTS OF HA NANOPARTICLES ON DEVITRIFICATION CRYSTALLIZATION OF PEG-600 CRYOPROTECTANTS

作　　者：吕福扣[1] 刘宝林[1] 李维杰[1] 

机构地区：[1]上海理工大学生物热科学研究所,上海200093

出　　处：《低温物理学报》2012年第4期315-320,共6页Low Temperature Physical Letters

基　　金：国家自然科学基金(批准号:51076108);教育部新世纪优秀人才计划(批准号:07-0559);上海市东方学者计划资助课题~~

摘　　要：为了研究羟基磷灰石HA纳米微粒对低温保护剂反玻璃化结晶的影响,本文利用DSC和低温显微镜研究了含有不同粒径(20nm、40nm、60nm)和不同质量浓度(0.1%、0.2%、0.4%、0.8%)HA纳米微粒的PEG-600(50%,w/w)溶液反玻璃化过程中的结晶现象.试验结果表明:与未添加纳米微粒的PEG-600溶液相比,加入40nm、0.4%纳米微粒的HA-PEG600溶液的反玻璃化温度升高了7℃;加入20nm、0.4%和40nm、0.8%纳米微粒的HA-PEG600溶液的冰晶生长速率分别降低了35%和提高了50%;纳米低温保护剂溶液的冰晶形貌从大圆形变成了小圆形、枝晶或小圆形中夹带枝晶.To study the effects of HA nanoparticles on devitrification of cryoprotectant solutions, The crystallization of PEG-600（50H）solutions with HA nanopartieles of different particle size（20nm.40nm. 60nm）and concentrations（0. 1%.0. 2%. 0. 4%. 0. 8%）were observed under a cryomicroscope, and vitrification parameters were measured by DSC. Experimental results showed that devitrifieation temperature of PEG-600 solution containing 0. 4H HA with the particle size of 40nm increased by 7℃, the growth rate of ice crystal of PEG-600 solution containing 0. 4% HA with the particle size of 20nm and 0. 8% HA with the particle size of 40nm decreased by 35% and increased by 50% respectively. The structure of ice crystal in PEG-600 solution with HA changed remarkably.

关 键 词：DSC 低温显微镜 反玻璃化温度 冰晶生长速率 冰晶形貌 

分 类 号：TQ171.1[化学工程—玻璃工业]