基于小鼠表达谱芯片和比较基因组学分析猪生长发育相关候选基因集  

Analysis of the candidate gene sets correlated to the growth and development of pigs based on a mouse expression microarray and the comparative genomics

作　　者：王琼萍[1,2] 杨帆[1,2] 王明辉[1,2] 何侃[1,2] 俞沛初[1,2] 潘玉春[1,2] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海市兽医生物技术重点实验室,上海200240

出　　处：《黑龙江畜牧兽医》2012年第8期145-147,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(N30871728)

摘　　要：为了在更宽、更深的层面上研究猪生长发育机制,得到以通路为单位的候选基因集以克服传统单基因分析方法的缺点。试验利用模式生物小鼠中的芯片技术,通过基因集富集分析方法(GSEA法)分析基因芯片,确定候选通路。对候选通路成员与猪生长发育相关的数量性状基因座(QTL)数据库进行映射,缩小其作用跨度区域,找出作用的关键基因集。结果表明:得到了56个与猪生长发育相关的候选基因集。To study the mechanisms of the growth and development of pigs at a wider and deeper level, and to get the candidate gene set using the pathway as a unit to overcome the shortcomings of traditional single gene analysis. The technology of Model Organism （mouse） ChIP -on -chip Microan＇ays was applied, and the gene microarray was analyzed using the gene set enrichment analysis method to identify candidate path- ways. Then, the members of candidate pathways were mapped with the quantitative trait loci （QTL） database correlated to the growth and de- velopment of pigs, which was for decreasing the span area to identify the key gene sets. The results showed that fifty six sets of candidate genes were obtained, which related with the growth and development of pigs.

关 键 词：候选基因集 数量性状基因座(QTL) 生长发育性状 

分 类 号：S828[农业科学—畜牧学]