氯化汞防治组培苗污染的研究  

Research on the Prevention of Tissue Culture Seedling Pollution by Mercuric Chloride

作　　者：彭广霖[1] 于咏梅[1] 薛元霞[1] 张志芬[1] 史淑一[1] 

机构地区：[1]中国农业大学烟台研究院,山东烟台264670

出　　处：《北方园艺》2012年第15期131-133,共3页Northern Horticulture

基　　金：中国农业大学(烟台)校级课题资助项目(yt2008.16)

摘　　要：以大花萱草和切花菊组培苗为试材,在MS培养基中分别加入0.005、0.010、0.030、0.050、0.070mg/L氯化汞,研究抑菌剂氯化汞对污染组培苗的消除污染效果。结果表明:当浓度在0.010mg/L时,经过2、3次的转接后,能明显地消除污染。当浓度在0.030mg/L时,1次转接即能对污染起到较好的抑制效果,并且随浓度的增加,抑制效果越好。但浓度超过0.080mg/L时,组培苗迅速出现脱绿死亡现象。综合分析认为,氯化汞的浓度在0.030～0.070mg/L是防治大花萱草和切花菊组培苗污染的最佳浓度。Chosen hemerocallis and cut chrysanthemum seedlings as materials, mercuric chloride at the rate of O. 005, 0. 010,0. 030,0. 050 and 0. 070 mg/L was added into culture medium, respectively, the effects of mercuric chloride on the pollution prevention of tissue culture seedling were studied. The results showed that mercuric chloride could significantly eliminate pollution at 0. 010 mg/L concentration by 2 to 3 switches. As mercuric chloride contents were between 0. 030 and 0. 070 mg/L,the pollution was markedly inhibited and a positive relationship was found between inhibition effect and concentrations. 0. 080 mg/Lmercuric chloride was unfavorable for tissue culture seedlings indicted by leaf chl0rosis and plant death. It was concluded that concentrations between 0. 030 and 0. 070 mg/L may be the better choice to prevent pollution for hemerocallis and cut chrysanthemum seedlings.

关 键 词：污染组培苗 氯化汞 消除污染 

分 类 号：Q943.1[生物学—植物学]