傅里叶变换中红外光谱法检测脑胶质瘤  

Investigation on Glioma Using FT-mid-IR Spectroscopy

作　　者：潘庆华[1,2] 万伟庆[3] 贾桂军[3] 徐怡庄[1] 来国桥[2] 华小辉[1] 刘少轩[1] 陈静[1] 杨丽敏[1] 张元福[1] 翁诗甫[1] 吴瑾光[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871 [2]杭州师范大学有机硅化学与材料重点实验室,杭州310012 [3]首都医科大学附属北京天坛医院,北京100083

出　　处：《高等学校化学学报》2012年第8期1703-1707,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:30371604,50203001,20171004);国家“八六三”计划项目(批准号:2010AA03A406)资助

摘　　要：采用傅里叶变换衰减全反射中红外光谱法检测了19例液氮冻存的脑胶质瘤离体组织样品(星形细胞瘤10例,少枝-星形细胞瘤9例),对得到的红外光谱进行分析发现,恶性程度不同的星形细胞瘤组织的红外光谱存在差异,并且不同类型的脑胶质瘤组织的红外光谱也表现出较为明显的区别,因此可以根据各个特征吸收峰的峰位、峰形及谱峰强度等信息来区分脑胶质瘤,并初步鉴别脑胶质瘤的性质.研究结果表明,通过某些特征吸收峰峰位的变化来鉴别星形细胞瘤和少枝-星形细胞瘤与病理诊断结果的符合率约为80%,说明傅里叶变换衰减全反射中红外光谱法有望发展成为一种对样品无损伤、快速的脑肿瘤诊断新方法.Nineteen cases of glioma tissues（including 10 cases of astrocytic tumors and 9 cases of oligoastrocytic tumors） were detected using NF-mid-IR spectroscopy linked with attenuated total reflectance （ATR） accessory. In the astrocytic tumors cases, there were 4 cases of pure astrocytic tumors （WHO I and II ） , 2 cases of anaplastic astrocytic tumors（ WHO III ） and 4 cases of glioblastoma（ WHO IV）. The FTIR spectra obtained from the brain tumor tissues were analyzed and compared. Significant differences were found in the peak position, band shape and intensity of infrared absorption spectra between the different grades of astrocytic tumors. The intensity of the band at ca. 1400 cm-1 band is increased with the malignancy grade of astrocytic tumors. In addition, some spectral differences between astrocytic tumors and oligoastrocytic tumors were observed. In the spectra of oligoastrocytic tumors, the peak position of 1240 and 1300 cm-1 shifted to low wavenumbers. And the band at ca. 1270 cm-1 appeared in the spectra of oligoastrocytic tumors which was not observed in the astrocytic tumors. These results demonstrate that FT-mid-IR spectroscopy exhibits prospect to develop a novel, non-invasive and rapid method for the detection of brain tumors.

关 键 词：傅里叶变换中红外光谱法 衰减全反射 胶质瘤 

分 类 号：O657.33[理学—分析化学]