十字花科植物HSP22基因同源序列的克隆与进化分析  

Cloning and evolutionary analysis of homologous sequences of HSP22 gene in Cruciferae

作　　者：程影[1,2] 黄锐之[3,4] 钟新民[2] 侯喜林[1] 吴学龙[3,4] 王五宏[2] 李必元[2] 刘智宏[3,4] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [2]浙江省农业科学院蔬菜研究所 [3]浙江省农业科学院病毒学与生物技术研究所 [4]浙江省植物代谢基因工程试验基地,浙江杭州310021

出　　处：《南京农业大学学报》2012年第4期41-46,共6页Journal of Nanjing Agricultural University

基　　金：国家863计划项目(2008AA02Z103);浙江省农业科学院科技创新项目

摘　　要：采用PCR克隆技术,根据已报道的拟南芥的AtHSP22基因全长及其他物种同源序列设计特异引物,在十字花科植物大白菜、普通白菜、芥菜型油菜、甘蓝型油菜、花椰菜、芥菜、荠菜、萝卜3个属10个物种中成功克隆得到耐热相关基因HSP22的同源序列10条。序列比对分析表明:这些同源序列的相似性达89.3%以上,所推导的氨基酸序列相似性达92.1%以上。由进化树可直观地看出,芸薹属与荠属亲缘关系最近,首先聚为一类;其次与萝卜属、拟南芥属聚为一类。由氨基酸序列预测的高级结构存在明显差异,且无规则卷曲最多,α-螺旋均在30%以上,因而属于α结构蛋白。HSP22 gene analogues from Brassica rapa subsp.pekinensis,Brassica chinensis,Raphanus sativus L.,Brassica rape,Brassica oleracea L.var.botrytis L.,Capsella bursa-pastoris L.,Brassica juncea Coss and Brassica napus,10 species of 3 genera in Cruciferae were obtained by PCR strategy using gene specific primers designed based on the full-length sequence of previously reported AtHSP22 gene and other similar sequences in homologous species.The secondary structure and three-dimensional structure were also predicted.Sequences alignment indicated that the similarities of the genes were more than 89.3% and 92.1% at nucleotide and amino acid levels,respectively.The NJ（Neighbor-joining）evolutionary tree showed that Brassica was closely related to Capsella,followed by Raphanus and Arabidopsis.The senior structures predicted by amino acid sequences displayed marked differences,the most content was random coil,and α-helix all reached to 30%,so it belongs to α structural proteins.

关 键 词：十字花科植物 HSP22基因 克隆 进化分析 

分 类 号：S63[农业科学—蔬菜学]