胆酸钠对山羊鼻腔相关扁桃体黏膜上皮吸收灭活病毒的影响  

Influence for absorption of inactivated virus in the epithelium of nasal-associated tonsil of goat by sodium cholate

作　　者：李正平[1] 杨倩[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2012年第4期94-98,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31172302);教育部博士点基金项目(B200606);江苏省科技支撑计划项目(BE200830155)

摘　　要：为研究胆酸钠促进山羊鼻腔相关扁桃体黏膜上皮吸收禽流感灭活病毒的形态学机制,经鼻腔接种胆酸钠和禽流感灭活病毒后,用组织学光镜和电镜制片技术研究山羊咽扁桃体和咽鼓管扁桃体上皮结构的变化以及灭活病毒的吸收情况。结果表明:单独应用灭活病毒后扁桃体黏膜上皮结构完整;而应用胆酸钠或胆酸钠配合灭活病毒后扁桃体中可观察到较多脱离黏膜上皮的细胞,脱落的细胞大部分是上皮细胞和淋巴细胞,上皮细胞间间隙增大,紧密连接被打开。应用胆酸钠配合灭活病毒后可观察到部分细胞内含有病毒的囊泡。鼻腔接种后第3天,应用胆酸钠的扁桃体黏膜上皮结构基本恢复,几乎没有观察到脱离上皮的细胞。结论:胆酸钠通过打开山羊鼻腔相关扁桃体黏膜上皮细胞之间的紧密连接来促进灭活病毒的吸收;虽然胆酸钠可引起部分上皮脱落,但这种副作用是短暂的并是可修复的。Mechanisms for absorption enhancement of inactivated avian influenza virus（H9N2）in the epithelium of nasal-associated tonsil of goat by sodium chalate was studied.Goats were immunized by intranasal with inactivated avian influenza virus which had been added a final concentration of 10 mg·mL-1 sodium cholate.The changes of the epithelial structure and the absorption of inactivated virus on the pharyngeal tonsil and tubal tonsil were studied by histology method and electron microscopy technique.The result was as follows：after intranasal immunization with inactivated virus,the structure of the epithelium was integral,but after intranasal immunization with sodium cholate or sodium cholate together with inactivated virus,a large number of falling cells were observed at the free face of the epithelium,most of the falling cells were epithelial cells or lymphocytes,and there was some gaps between the epithelial cells.After intranasal immunization with sodium cholate together with inactivated virus,some vesicles which contained inactivated virus were found in few epithelial cells.At 3 days after intranasal immunization with sodium cholate,the structure of the epithelium was recovered.Conclusion：the result showed that the sodium cholate could open the tight junction between the epithelial cells of the nasal-associated tonsil to enhance the absorption of inactivated virus,although it could cause some epithelial cells falling off,this effect was temporary and could be recovered in a short time.

关 键 词：胆酸钠 山羊 扁桃体 灭活病毒 

分 类 号：S852.16[农业科学—基础兽医学]