恩诺沙星在异育银鲫体内的组织分布及消除规律  

Distribution and elimination of enrofloxacin in allogynogenetic silver crucian carp,Carassius auratus gibelio

作　　者：方星星[1] 刘秀红[1] 丁相卿[1] 赵文婷[1] 刘文斌[1] 陆承平[2] 

机构地区：[1]南京农业大学动物科技学院 [2]南京农业大学动物医学院,江苏南京210095

出　　处：《南京农业大学学报》2012年第4期110-114,共5页Journal of Nanjing Agricultural University

基　　金：农业部水生动物遗传育种和养殖生物学重点开放实验室开放基金(BZ2007-04)

摘　　要：在24～26℃水温条件下,以10 mg.kg-1剂量,用高效液相色谱法检测组织中药物浓度,研究静脉注射和口服给药后恩诺沙星在健康异育银鲫组织内的代谢分布规律。结果表明:静脉注射后,药物在组织中代谢动力学特征符合二室模型;口服给药后,药物吸收良好,生物利用度(F)为86%,组织药物浓度-时间曲线呈现双峰,推测是由于药物在异育银鲫体内的肠肝循环作用所致。静脉注射和口服两种给药方式下,恩诺沙星在异育银鲫体内均具有良好的组织分布,肾脏、肌肉、肝胰脏、鳃和血液5种组织中的药物浓度时间曲线下总面积(AUC)分别为624.2、965.9、721.8、298.0、239.6μg·h·mL-1和465.3、343.1、542.9、411.4、205.9μg·h·mL-1,最大药物浓度(Cmax)分别为33.48、16.91、26.44、18.71μg.g-1和15.30μg·mL-1,9.20、5.39、7.78、6.88μg.g-1和4.50μg·mL-1;药物在各组织中消除时间较长,消除半衰期(T1/2β)分别为169.0、141.4、113.4、36.7、63.5 h和27.3、49.2、77.0、38.5、62.7 h。结论:恩诺沙星以10 mg.kg-1剂量单次口服给药,对细菌引起的异育银鲫病可以起到较好的治疗作用,但需注意药物残留问题。The distribution of enrofloxacin（EF）in tissues was investigated after single intravenous（i.v.）and oral（p.o.）administration of 10 mg·kg-1 body weight in allogynogenetic silver crucian carp at 24-26 ℃.The tissue concentration of EF was determined by high-performance liquid chromatography（HPLC）.A two-compartment model kinetics was observed following i.v.administration.The plasma concentration-time data following p.o.administration showed a double peak-shaped curve,indicating the possibility of enterohepatic recirculation of EF in allogynogenetic silver crucian carp.The absorption of EF was very good with a bioavailability（F）of 86%.After both administrations,the distribution of drug was extensive,the AUC in kidney,muscle,Hepatopancreas,gill and blood was 624.2,965.9,721.8,298.0,239.6 μg·h·mL-1 and 465.3,343.1,542.9,411.4,205.9 μg·h·mL-1,respectively;the Cmax in five tissues was 33.48,16.91,26.44,18.71 μg·g-1 and 15.30 μg·mL-1,9.20,5.39,7.78,6.88 μg·g-1 and 4.50 μg·mL-1,respectively.The depletion time of EF was considerably long,the T1/2β of drug in five tissues was 169.0,141.4,113.4,36.7,63.5 h and 27.3,49.2,77.0,38.5,62.7 h,respectively.Conclusion：EF administered p.o.at a dose of 10 mg·kg-1 might offer a good option for the treatment of infectious diseases in allogynogenetic silver crucian carp,while its relatively long T1/2β indicated a long withdrawal period.

关 键 词：恩诺沙星 异育银鲫 组织分布 消除规律 

分 类 号：S948[农业科学—水产养殖]