结球甘蓝-大白菜异源三倍体与结球甘蓝回交获得初级三体的研究  

Production of a primary trisomic from progeny of cabbage-Chinese cabbage allotriploid backcrossing with cabbage

作　　者：李艳霞[1] 管志坤[1] 赵建军[1] 申书兴[1] 王彦华[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071000

出　　处：《河北农业大学学报》2012年第4期37-40,共4页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金(C2010000677)

摘　　要：采用结球甘蓝-大白菜异源三倍体与二倍体结球甘蓝回交的方式,从回交后代BC2中获得了1株2n+1非整倍体植株。经细胞学和SSR鉴定分析,确认该2n+1植株为结球甘蓝8号初级三体。通过对8号初级三体植株自交和回交后代染色体数目的测定,明确了其额外染色体雌、雄配子的传递率分别为34.74%和6.17%。In this study, an aneuploid plant with 2n＋ 1 chromosome was obtained from the progeny of cabbage-Chinese cabbage allotriploid backcrossing with cabbage. Cytological and SSR analysis showed that the aneuploid plant was a primary trisomic - 8 of cabbage. Furthermore, the chromosome number of the selfing and backcrossing progenies of the primary triso- mic-8 plant was identified, and the transfer rate of female and male gametes of extra-chromo- some was clarified as 34.74% and 6.17%, respectively.

关 键 词：结球甘蓝 异源三倍体 初级三体 核型分析 SSR 

分 类 号：S634.1[农业科学—蔬菜学] S635.1[农业科学—园艺学]