蛔虫体腔液对3T3-L1细胞分化和凋亡的作用  

Effects of ascaris body cavity fluid on differentiation and apoptosis of 3T3-L1 preadipocytes

作　　者：褚军[1] 薛晓阳[1] 刘颖[1] 陈立功[1] 张宝贵 董世山[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北省永清县畜牧兽医局,河北永清065600

出　　处：《河北农业大学学报》2012年第4期100-103,共4页Journal of Hebei Agricultural University

基　　金：河北省科技支撑计划项目:高通量筛选抗病毒中兽药活性成分样品库建立及禽类重大传染病的防控(07220401D-01)

摘　　要：为了探讨蛔虫体腔液(Ascaris body cavity fluid,ABF)对小鼠3T3-L1前脂肪细胞分化过程的作用,揭示ABF对机体脂类代谢的影响。本试验采用半定量聚合酶链式反应技术(RT-PCR)检测前脂肪细胞分化相关基因过氧化物酶体增殖剂活化受体γ(Peroxisome proliferator-activated receptorγ,PPARγ)、GAPDH mRNA的表达;流式细胞技术(FCM)检测ABF对3T3-L1细胞凋亡的影响。结果表明:1 000μg/mL的ABF明显降低前脂肪细胞分化相关基因PPARγmRNA的相对表达,与对照相比,差异极显著(P<0.01)。在3T3-L1分化过程中加入1 000μg/mL ABF可增加细胞的凋亡。表明一定浓度的ABF对3T3-L1前脂肪细胞分化具有明显的抑制作用,并且能够诱导3T3-L1前脂肪细胞分化过程中的细胞凋亡,提示ABF能够抑制前脂肪细胞转化为脂肪细胞,可能具有调控脂类代谢以及控制高脂血症相关疾病的潜力。To investigate the effects of Ascaris body cavity fluid（ABF） on differentiation and apoptosis of 3T3-L1 preadipocytes, the expressions of PPAR7 mRNA and GAPDH mRNA were measured by quantitative real time PCR. Apoptosis rates in the stage of differentiation in 3T3-L1 cells were detected by flow cytometric analysis before and after the treatment of Ascaris body cavity fluid. The results showed that 1 000μg/mL ABF significantly reduced mRNA expressions of PPART. The apoptosis rate was increased during 3T3-L1 differentiation by adding 1 000μg/mL ABF. 1 000 μg/mL ABF had a significant cytotoxic effect on 3T3-L1 prea- dipocytes, and significantly induced apoptosis. ABF inhibited the differentiation of preadipoeytes into adipocytes, and may have the potential of regulation of lipid metabolism and control of hyperlipidemia.

关 键 词：蛔虫体腔液 前脂肪细胞 过氧化物酶体增殖物激活受体 凋亡 

分 类 号：S642.2[农业科学—蔬菜学]