山豆根不同组分抗炎及伴随毒副作用研究  

Study on anti-inflammatory efficacy accompanied by side effects of different components of Sophorae Tonkinensis Radix et Rhizoma

作　　者：李晓宇[1,2] 栾永福[3] 李晓骄阳 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355 [3]天津中医药大学,天津300073 [4]中国药科大学药学院,江苏南京211198

出　　处：《中国中药杂志》2012年第15期2232-2237,共6页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2009CB522802);山东省科技平台建设项目(2008GG2NS02021)

摘　　要：目的:考察并比较山豆根不同组分发挥抗炎药效剂量下的伴随毒副作用及其机制进行研究,阐释有毒中药"毒性-药效"相关性。方法:运用小鼠耳肿胀和肉芽肿模型,给小鼠灌胃不同剂量的山豆根水提和醇提组分,观察其在发挥药效作用时的伴随毒副作用,同时检测血清ALT,AST的活性,SOD,MDA,PEG2,NO,NOS,Cr,BUN,GSH,TG,Gn含量的变化及脏器指数。结果:药效剂量的山豆根水提和醇提组分对巴豆油小鼠耳肿胀和琼脂肉芽肿均有一定的肿胀抑制作用,水提组分和醇提组分药后3 d和药后8 d山豆根可使血清ALT,AST活性升高,SOD,MDA,PEG2,NO,NOS,GSH,TG,Gn等指标均有明显变化。结论:药效剂量的山豆根水提和醇提组分具有较强的抗炎作用,同时会产生伴随副作用和肝毒性,不同组分之间作用强度有异。不同组分抗炎作用机制与其减少炎性介质释放、抑制过氧化损伤有关;山豆根抗炎同时产生一定的肝毒性,过氧化损伤可能是其损伤机制之一。Objective： To investigate the anti-inflammatory efficacy accompanied by side effects of water extract and alcohol extract of Sophorae Tonkinensis Radix et Rhizome（STRR）,their molecular mechanism,and interpret the relationship of ＂toxicity-effect＂ of toxic medicine.Method： The ear swelling by croton oil and granuloma by agar test models were used,water extract and alcohol extract of STRR of different dosages were administrated ig to mice to observe the assident toxicity,at the same time the activities of ALT,AST and the content of SOD,MDA,PEG2,NO,NOS,Cr,BUN,GSH,TG and Gn in serum were tested.Result： Both water extract and alcohol extract of STRR have a strong inhibitory effect on ear swelling by croton oil and granuloma by agar.The activities of ALT,AST in serum were higher than that of normal group.SOD,MDA,PEG2,NO,NOS,GSH,TG and Gn had obvious changes.Conclusion： Both water extract and alcohol extract of STRR had an anti-inflammatory effect on acute and chronic inflammation.At the same time,side effects and liver toxicity.The anti-inflammatory effect of STRR in probable relation to the reduced inflammatory mediators release.Oxidative damnification might be one of the liver injury mechanism.

关 键 词：山豆根 水提和醇提组分 小鼠 抗炎 毒副作用 

分 类 号：R285.5[医药卫生—中药学]