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作 者:何报作[1] 朱意麟[1] 廖月葵[1] 黎理[1] 周媛[1] 袁正伟[1] 曾静[1]
出 处:《中国中药杂志》2012年第15期2262-2266,共5页China Journal of Chinese Materia Medica
基 金:国家自然科学基金项目(30860371)
摘 要:目的:寻找磨盘草与其易淆品苘麻的鉴别特征,建立有效的鉴别方法。方法:LMVP(叶形态-脉序图谱鉴别法),QAERM(定量分析评价鉴别中药品种真伪方法的可靠性的方法)。结果:主要区别点为磨盘草叶的睫毛短(75~200μm)、不易察见,叶柄上端具较长的展开毛(长达1.0~1.2 cm),中脉下部的三级脉或间脉的夹角多为宽锐角,少近直角;苘麻叶的睫毛较长(200~500μm)、易察见,叶柄上端具较短的展开毛(多长0.1~0.5 mm),中脉下部的三级脉或间脉的夹角多为近直角,少宽锐角。同时以上述3组区别点为指标,鉴别结果的正确性(Ac)97.5%~99.6%;重复性为符合率(ARO)97.1%,一致性强度(Kappa)0.94。结论:建立的"叶形态-脉序图谱鉴别法"可准确鉴别磨盘草及易淆品苘麻。操作简便、快速、价廉、可靠性强。Objective: To study the identification characters of Abutilon indicum and its confused herb A.theophrasti and establish an identification method.Method: LMVP(leaf morphological-venation pattern for identification Chinese herbs),and QAERM(quantitatively analyze and evaluate reliability for the method of identification Chinese herbs) were applied for the study.Result: The main differences of the leaf of Abutilon indicum: leaf margin cilia short and not clear(75-200 μm),petiole top with long straight hairs(up to 1.0-1.2 cm),when the tertiary veins and intersecondary veins issue from the lower part of midrib,their angles of divergence usually were wide acute(65°-80°) and a few was about right angle(80°-100°).The main differences of the leaf of A.theophrasti: leaf margin cilia long and clear(200-500 μm),petiole top with short straight hairs(most 0.1-0.5 mm),when the tertiary veins and intersecondary veins issue from the lower part of midrib,their angles of divergence usually were about right angle(80°-100°) and a few was wide acute(65°-80°).With the mentioned three groups of key differences,the both plants could be successfully identified from each other.The accuracy of identification results(AC) was from 97.5% to 99.6%,the repeatability of identification results: agreement rate for observation(ARO) was 97.1% and Kappa value was 0.94.Conclusion: The established method is simple,rapid,economic and reliable.
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