白色念珠菌分泌型天冬氨酸蛋白酶在口腔扁平苔藓患者唾液中的表达  

Expression of Candida albicans Secretory Aspartyl Proteinases in the Saliva of Oral Lichen Planus Patients

作　　者：张英[1] 闻妍[1] 康媛媛[1] 钟鸣[2] 

机构地区：[1]中国医科大学口腔医学院综合急诊科 辽宁省口腔医学研究所口腔黏膜病学研究室,辽宁沈阳110002 [2]中国医科大学口腔医学院中心实验室,辽宁沈阳110002

出　　处：《微生物学杂志》2012年第4期40-46,共7页Journal of Microbiology

基　　金：辽宁省自然科学基金(20092115)

摘　　要：研究白色念珠菌分泌型天冬氨酸蛋白酶(SAP)家族在扁平苔藓患者唾液中的表达,进而探讨白色念珠菌病与扁平苔藓的关系。选取白色念珠菌携带者、白色念珠菌病患者、携带白色念珠菌的扁平苔藓患者、伴有白色念珠菌病的扁平苔藓患者各20例,念珠菌培养阴性的扁平苔藓患者10例。从唾液标本中直接提取RNA,进行RT-PCR。白色念珠菌携带者唾液中有SAP2、SAP4-6、SAP7的不同表达,携带白色念珠菌的口腔扁平苔藓患者唾液中有SAP2、SAP4-6、SAP7、SAP8、SAP9的不同表达,所有携带者中均未表现SAP1、SAP3和SAP10的阳性扩增。白色念珠菌患病者及伴有白色念珠菌病的口腔扁平苔藓患者唾液中有SAP1-10的不同表达。念珠菌培养阴性的扁平苔藓患者均无SAP1-10的阳性扩增。SAP9与扁平苔藓密切相关。Expressions of secretory aspartyl proteinase （SAP） family of Candida albicans in oral lichen planus （OLP） patients was studied to reveal the relation between OLP and C. albicans infections. The carriers of C. albicans and eandidiasis patients, OLP patient with C. albicans carriers, and OLP patients accompanied withcandidiasis 20 cases each, and 10 cases of OLP patients with C. albicans culture negative were selected. The RNA directly extracted / from saliva was carried out RT-PCR. There were different expressions of SAP2, $AP4-6, and SAP＇7 in saliva of C. al- bicans carriers, and there were different expressions of SAP2, SAP4-6, SAP＇/, SAP8, and SAP9 in saliva of OLP pa- tient with C. albicans carriers, positive amplification of SAPI, SAP3, and SAP10 were not found in all carriers. There were different expressions of SAPI-10 in saliva of candidiasis patients and OLP patients accompanied with can- didiasis. OLP patient with C. albicans culture negative had no SAPI-10 positive amplification. SAP9 had close corre- lation to oral lichen planus.

关 键 词：白色念珠菌 分泌型天冬氨酸蛋白酶 扁平苔藓 

分 类 号：Q556.9[生物学—生物化学]