Modeling of the fibrin agarose plate assay and its application for thrombolytic analysis  

作　　者：WANG MingQi YANG WenZhi WU Qiang GU HongChen 

机构地区：[1]Nano Biomedical Research Center,School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University,Shanghai 200030,China [2]Department of Ophthalmology,The Sixth People's Hospital,Shanghai Jiao Tong University,Shanghai 200233,China

出　　处：《Chinese Science Bulletin》2012年第24期3233-3238,共6页

基　　金：supported by the SJTU Program (YG2009ZD203)

摘　　要：The fibrin agarose plate assay is widely used in the detection of thrombolysis efficacy. However, a rigorous mathematical model for analyzing data or comparing activities of different thrombolytics has been absent. This study investigated the relationship between thrombolysis radius, R, and diffusion time, t, of molecular medicines in an agarose hydrogel system by deriving a model based on Fick's law and experimental verification by the fibrin agarose plate assay method. The theoretical results showed that a plot of log(R) versus log(t) has a linear curve with the slope of 1/2 and this was verified by experimental results using urokinase as a modeling agent. Moreover, it was found that R÷t is constant for a specific thrombolytic and can be used as a parameter for evaluating activities of different thrombolytics. The theoretical model has potential for improving the understanding of mecha-nisms involved in molecular medicine diffusion and offers benefits for thrombolytic therapy.The fibrin agarose plate assay is widely used in the detection of thrombolysis efficacy. However, a rigorous mathematical model for analyzing data or comparing activities of different thrombolytics has been absent. This study investigated the relationship between thrombolysis radius, R, and diffusion time, t, of molecular medicines in an agarose hydrogel system by deriving a model based on Fick＇s law and experimental verification by the fibrin agarose plate assay method. The theoretical results showed that a plot of log（R） versus log（t） has a linear curve with the slope of 1/2 and this was verified by experimental results using urokinase as a modeling agent. Moreover, it was found that is constant for a specific thrombolytic and can be used as a parameter for evaluating activities of different thrombolytics. The theoretical model has potential for improving the understanding of mecha-nisms involved in molecular medicine diffusion and offers benefits for thrombolytic therapy.

关 键 词：琼脂糖凝胶 纤维蛋白 平板试验 溶栓 建模 应用 Fick定律 数学模型 

分 类 号：R911[医药卫生—药学]