可感染茶尺蠖的重组家蚕核型多角体病毒的构建  

Construction of Recombinant BmNPV Infecting Ecotropis oblique

作　　者：郭睿[1] 刘卫星[1] 潘中华[1] 薛仁宇[1] 曹广力[1] 朱越雄[1] 贡成良[1] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123

出　　处：《中国农业科学》2012年第16期3288-3296,共9页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(31072085);江苏省自然科学基金项目(BK2009117);江苏省"六大人才高峰"项目(2009-D-Ag18);苏州市自然科学基金项目(YJG0907);苏州大学重大应用研究培育项目(Q3134991)

摘　　要：【目的】构建能够感染家蚕和茶尺蠖的重组家蚕核型多角体病毒(rBmNPV),解决EoNPV扩增困难而不易获得的难题。【方法】通过Bac-to-Bac系统构建具备BmNPV的多角体蛋白基因(polh)表达盒和EoNPV的解旋酶基因(hel)表达盒的重组家蚕核型多角体病毒。【结果】家蚕或家蚕细胞扩增的rBmNPV可以感染茶尺蠖并致病,表明rBmNPV的宿主域拓展至茶尺蠖。【结论】成功构建了能够感染茶尺蠖的rBmNPV,为利用家蚕作为宿主来生产重组病毒杀虫剂打下基础。[ Objective ] The objective of this study is to construct recombinant BmNPV infecting both silkworm and Ecotropis oblique, and to solve the reproduction difficulty of EoNPV because of the breeding complexity. [Method] Recombinant BmNPV containing the polh expression box of BmNPV and the hel expression box of EoNPV were constructed by Bac-to-Bac system. [Result] rBmNPV amplified from silkworm or BraN cell could infect E. oblique, which indicated the host domain of rBmNPV had been broaden to E. oblique. [Conclusion] rBmNPV which could infect E. oblique was successfully constructed, thus laied a foundation of producing recombinant virus pesticide using silkworm as host.

关 键 词：EoNPV BMNPV 重组病毒 宿主域 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]