疣状毛癣菌感染豚鼠模型的构建  

Establishment of a guinea pig model of dermatophytosis caused by Trichophyton verrucosum

作　　者：李玲[1] 尤德渊[1] 

机构地区：[1]哈尔滨医科大学附属第二医院皮肤科,150086

出　　处：《中华皮肤科杂志》2012年第8期582-583,共2页Chinese Journal of Dermatology

摘　　要：目的构建疣状毛癣菌感染豚鼠的动物模型。方法选择健康豚鼠32只，随机分为4组。第1组未给任何处置，第2、4组豚鼠刮毛并摩擦其背部皮肤，第3组豚鼠背部给予刮毛处理。第1、2、3组为实验观察组，分别涂抹疣状毛癣菌菌悬液100μl；第4组涂抹须毛癣菌菌悬液100μl。采用直接镜检、真菌培养和组织病理方法验证感染结果。结果第1组直接镜检持续阴性；第2、3、4组豚鼠背部鳞屑直接镜检至第7-9天时均为阳性；将鳞屑接种于沙氏葡萄糖琼脂（SDA）平板上培养，7d后可见真菌生长，2周后各平板接种点均有菌落生长，且与接种前菌落形态基本相同。第2、3、4组取皮损活检HE染色，表皮角质层及部分毛囊漏斗部可见折光性强的菌丝及孢子。结论成功构建了疣状毛癣菌感染的豚鼠模型。Objective To establish a guinea pig model of dermatophytosis caused by Tdchophyton verrucosum. Methods Thirty-two guinea pigs were randomly and equally divided into four groups to remain untreated （group 1 ）, receive depilation and skin abrasion in the back （group 2 and 4）, or only depilation （group 3）. The back was smeared with a suspension （100 Ixl） of Tffehophyton verrucosum in group l, 2 and 3, and with that of Trichophyton mentagrophytes in group 4. Direct microscopy, fungal culture and histopathology were performed to validate the infection of guinea pigs by Tdchophyton. Results Direct microscopy was persistently negative in group l, turned to be positive from the 7th to 9th day in group 2, 3, and 4. Colonies began to appear 7 days after the inoculation of scrapings onto Sabouraud＇s dextrose agar （SDA） in group 2 and 3, and were observed at all the inoculated sites 2 weeks after the inoculation, with a morphological similarity to Trichophyton verrucosum before inoculation into guinea pigs. Hematoxylin and eosin staining revealed strongly refractive hyphae and spores in stratum comeum epidermidis and infundibular regions of some hair follicles in group 2 and 3. Conclusion A guinea pig model of dcrmatophytosis caused by Tdchophyton verrucosum is successfully constructed.

关 键 词：疣状毛癣菌感染 豚鼠模型 直接镜检 菌落生长 接种点 动物模型 实验观察 组织病理 

分 类 号：R756[医药卫生—皮肤病学与性病学] R-332[医药卫生—临床医学]