电针内关、心俞穴对急性心肌缺血大鼠脊髓背根神经电活动及下丘脑室旁核去甲肾上腺素、多巴胺浓度的影响  

Effects of electroacupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia

作　　者：李梦[1] 胡玲[1] 蔡荣林[1] 吴子建[1] 汪克明[1] 

机构地区：[1]安徽中医学院针灸经络研究所,安徽合肥230038

出　　处：《中西医结合学报》2012年第8期874-879,共6页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.81102659);安徽省高等学校自然科学研究项目(No.KJ2011A188);针灸基础与技术安徽省重点实验室开放基金资助项目(No.2009zjkf001A)

摘　　要：目的:探讨电针内关、心俞穴对急性心肌缺血大鼠脊髓背根神经电活动及下丘脑室旁核去甲肾上腺素(norepinephrine,NE)和多巴胺(dopamine,DA)浓度的影响。方法:雄性Sprague-Dawley大鼠100只随机分为假手术组、模型组、电针心俞组、电针内关组和电针内关加心俞组,每组20只。除假手术组外,其余大鼠用于制备急性心肌缺血再灌注模型。电针针刺3d后,双极电极记录各组大鼠脊髓背根神经电活动,高效液相色谱法检测各组大鼠下丘脑室旁核微透析液中NE和DA的浓度。结果:与假手术组大鼠比较,模型组大鼠脊髓背根神经电活动频率与幅值均明显升高,下丘脑室旁核NE和DA浓度明显下降(P<0.01)。与模型组比较,电针内关组、电针心俞组及电针内关加心俞组的脊髓背根神经细束电活动均明显下降,下丘脑室旁核NE和DA浓度明显升高(P<0.01);与电针内关组、电针心俞组比较,电针内关加心俞组脊髓背根神经电活动下调明显,下丘脑室旁核NE和DA浓度显著升高(P<0.05)。结论:电针心俞、内关穴对大鼠急性心肌缺血具有明显的协调保护效应,其可能作用机制与脊髓背根神经电活动、下丘脑内单胺类神经递质有关。OBJECTIVE： To investigate the effects of electroacupuncture （EA） at Neiguan （PC6） and Xinshu （BL15） on the nerve electrical activity in spinal dorsal root and norepinephrine （NE） and dopamine （DA） concentrations in the paraventricular nucleus of the hypothalamus in rats with acute myocardial ischemia （AMI）. METHODS： A total of ]00 male Sprague-Dawley rats were randomly divided into sham-operation, model, EA at PC6, EA at BL]5 and EA at both PC6 and BL15 groups with 20 rats in each group. The nerve electrical activity in spinal dorsal roots was recorded by bipolar electrodes. NE and DA concentrations in the paravent- ricular nucleus of the hypothalamus were detected by high-performance liquid chromatography. RESULTS： When compared with the sham-operation group, the nerve electrical activity in spinal dorsal roots was significantly increased while the NE and DA concentrations in the paraventricular nucleus of the hypothalamus were decreased in the model group （P〈0. 01）, The nerve electrical activity in spinal dorsal roots was decreased and the NE and DA concentrations were increased in the paraventricular nucleus of the hypothalamus in the EA at PC6 group, the EA at BL15 group and the EA at both PC6 and BLI5 group in comparison to those in the model group （P〈0.01）. The nerve electrical activity in spinal dorsal roots and the NE and DA concen- trations in paraventricular nucleus of hypothalamus of the EA at both PC6 and BL15 group were significantly improved when compared to those of the EA at PC6 and EA at BL15 groups （P^0.05）. CONCLUSION： EA at both PC6 and BL15 acupoints exhibits; the synergistic protective effects against AMI. The possible mechanism is related to regulating the nerve electrical activity in spinal dorsal roots and the concentrations of NE and DA in paraventricular nucleus of the hypothalamus.

关 键 词：穴 内关 穴 心俞 神经节 脊 去甲肾上腺素 多巴胺 心肌缺血 电针 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]