基于UPLC/Q-TOF-MS分析附子煎煮过程中化学成分的变化  

UPLC/Q-TOF-MS-based chemical profiling approach to evaluate the chemical constitution of Radix Aconiti Lateralis Preparata in the process of decoction

作　　者：周思思[1,2] 马增春[1] 梁乾德[1] 王宇光[1] 谭洪玲[1] 肖成荣[1] 张伯礼[3] 高月[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]安徽医科大学研究生学院,安徽合肥230032 [3]天津中医药大学,天津300193

出　　处：《中西医结合学报》2012年第8期894-900,共7页Journal of Chinese Integrative Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2011CB505304;No.2012CB518402);北京市自然科学基金资助项目(No.7112110)

摘　　要：目的:利用超高效液相-四级杆-飞行时间串联质谱技术(ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry,UPLC/Q-TOF-MS)分析附子随煎煮时间延长其生物碱含量的变化,从中药化学角度为附子炮制、含附子中药复方制备工艺等提供科学依据。方法:基于UPLC/Q-TOF-MS建立附子不同时间点水煎液的化学指纹图谱,通过主成分分析和偏最小二乘判别法分析附子随煎煮时间生物碱类成分的含量变化。结果:乌头碱、中乌头碱、次乌头碱等毒性较大的双酯型二萜类生物碱,在煎煮2～10min后含量较高,而苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰中乌头原碱等毒性较小的单酯型二萜类生物碱的含量在煎煮60min内是趋于增加的,随后其含量趋于稳定。结论:临床使用附子应根据适应证选择适宜的煎煮时间,避免煎煮不足导致中毒,或煎煮太过影响疗效。以双酯型二萜类生物碱为主要药效成分时,附子制备时间最好在30min内;以单酯型二萜类生物碱为主要药效成分时,附子制备时间最好在60min内。OBJECTIVE： An ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry （UPLC/Q-TOF-MS）-based chemical analytic technology was used to evaluate the chemical constitution of Radix Aconiti Lateralis Preparata in the process of decocting, so as to provide a scientific basis for processing Radix Aconiti Lateralis Preparata. METHODS： Radix Aconiti Lateralis Preparata samples with different decocting time were detected using UPLC/Q-TOF-MS to create a fingerprint spectrum. The data of t,-m/z pairs, ion intensities and sample codes were processed by partial least squared-discriminant analysis （PLS-DA） to holistically compare the difference among these samples. RESULTS： The contents of diester-diterpenoid alkaloid such as aconitine, mesaconitine and hypaconitine were higher between 2 to 10 min, while the contents of the lower toxic monoester-diterpenoid alkaloids such as benzoylaconine, benzoylmesaconine and benzoylhypaconine increased during the first 60 min, then their contents stabilized. CONCLUSION： Selecting the correct processing time for Radix Aconiti Lateralis Preparata according to clinical indications is essential for attenuating toxicity and increasing efficiency. Within 30 rain is the best time for diester-diterpenoid alkaloids as the main pharmacodynamic material, and within 60 min is the best time for monoester-diterpenoid alkaloids as the pharmacodynamic material of Radix Aconiti Lateralis Preparata.

关 键 词：附子 煎煮 液质联用 

分 类 号：R284[医药卫生—中药学]