熄风胶囊对氯化锂-匹罗卡品诱导的癫大鼠大脑皮质和海马多药耐药相关蛋白1表达的影响  

Effects of Chinese herbal medicine Xifeng Capsule on multidrug resistance-associated protein 1 expression in hippocampus and cortex of rats with lithium-pilocarpine-induced epilepsy

作　　者：李新民[1] 陈会[1] 任艳艳[1] 路岩莉[1] 

机构地区：[1]天津中医药大学第一附属医院儿科,天津300193

出　　处：《中西医结合学报》2012年第8期911-917,共7页Journal of Chinese Integrative Medicine

基　　金：天津市高等学校科技发展基金重点项目(No.2006ZD03);天津市社会发展计划应用基础研究面上项目(No.08JCYDJC10900)

摘　　要：目的:研究熄风胶囊单药及与卡马西平联合用药对氯化锂-匹罗卡品诱导的癫大鼠反复自发性发作及多药耐药相关蛋白1(multidrug resistance-associated protein1,MRP1)表达的影响。方法:应用氯化锂-匹罗卡品诱导难治性癫大鼠模型。将造模成功的癫大鼠随机分为模型组、熄风胶囊高剂量组、熄风胶囊中剂量组、熄风胶囊低剂量组、熄风胶囊高剂量加卡马西平组(高剂量联合组)、熄风胶囊高剂量加卡马西平1/2剂量组(低剂量联合组)和卡马西平组,每组10只,并选择10只正常大鼠作为对照。治疗28d后,取大脑皮质和海马组织,采用免疫组织化学方法检测癫大鼠大脑皮质与海马MRP1的表达。结果:各治疗组大鼠海马MRP1的表达均高于正常对照组,表达范围较广泛,阳性颗粒颜色较深,同时又低于模型组;在大脑皮质区,高剂量联合组、低剂量联合组与模型组比较差异有统计学意义(P<0.05);无论是在海马CA1、CA3区,齿状回及大脑皮质区,高剂量熄风胶囊对MRP1的分布的作用要优于低剂量熄风胶囊和中剂量熄风胶囊;与卡马西平联合用药时均优于低剂量熄风胶囊、中剂量熄风胶囊及卡马西平(P<0.05)。结论:熄风胶囊单药及与卡马西平联合用药能有效地抑制癫大鼠海马与皮质MRP1的过度表达。OBJECTIVE： To study the effects of Xifeng Capsule, a compound traditional Chinese herbal medicine, combined with carbamazepine on spontaneous epileptic seizure induced by lithium and pilocarpine in rats and the expression level of multidrug resistance-associated protein 1（MRP1）. METHODS： Lithium and pilocarpine were used to induce epilepsies in rats. All epileptic rats were randomly divided into model, high-dose Xifeng Capsule, medium-dose Xifeng Capsule, low-dose Xifeng Capsule, high- dose Xifeng Capsule plus carbamazepine （CBZ） （combined high-dose group）, high-dose Xifeng Capsule plus half dose of CBZ （combined low-dose group） and CBZ groups with 10 rats in each group. And another 10 normal rats served as control. After treating 28 d, immunohistochemical method was used to detect the MRP1 expression in cortex and hippocampus of the epileptic rats. RESULTS： MRP1 expression in hippocampus of the treated groups was higher than that of the normal control group, with wider range and darker positive particles, but was lower than that of the model group. In the cortical areas, the differences between the combined high-dose group or the combined low-dose group and the model group were statistically significant （P〈0.05）. Regardless of the hippocampusCA1, CA3, gyrus or cortical areas, the influence of high-dose Xifeng Capsule on MRPI distribution was superior to that of low- dose Xifeng Capsule; Xifeng Capsule combined with CBZ had better effects than low-dose Xifeng Capsule, medium-dose Xifeng Capsule and CBZ used alone （P〈0,05）. CONCLUSION： Xifeng Capsule used alone or combined with CBZ can effectively inhibit MRP1 expression in hippocampus and cortex of epileptic rats.

关 键 词：癫 中药 匹罗卡品 多药耐药相关蛋白1 大鼠 

分 类 号：R285.5[医药卫生—中药学]