毛白杨花粉败育过程中Ca^(2+)-ATPase的异常变化  

Anomalous changes in Ca^(2+)-ATPase distribution during the process of pollen abortion in Populus tomentosa Carr

作　　者：曹媛[1] 康向阳[1] 张志毅[1] 荆艳萍[1] 

机构地区：[1]北京林业大学生物科学与技术学院,林木育种国家工程实验室,林木花卉遗传育种教育部重点实验室

出　　处：《北京林业大学学报》2012年第4期10-17,共8页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(30700636)

摘　　要：为研究木本植物花粉败育过程中Ca2+-ATPase的影响,本文利用氯化铈沉淀和电镜细胞化学方法,对不同育性毛白杨花药在小孢子发生发育过程中Ca2+-ATPase进行了定位。结果表明:Ca2+-ATPase在可育花药小孢子母细胞时期大量分布于小孢子母细胞质膜、液泡膜及内膜系统,花药内壁细胞内膜系统和绒毡层细胞质膜,随后Ca2+-ATPase在上述细胞中均减少甚至消失,并于小孢子时期和成熟花粉时期分别在小孢子和花药内壁细胞中再次沉积;不育花药小孢子母细胞以及此时期绒毡层细胞质膜上并无明显Ca2+-ATPase,花药表皮、药室内壁以及中层的Ca2+-ATPase都高于同一时期的可育花药,其绒毡层细胞解体不完全。由上述结果推测:毛白杨不育花药内小孢子母细胞和药壁中Ca2+-ATPase分布异常,影响细胞内钙离子的主动转运,可能导致钙离子异常累积,进而通过影响小孢子母细胞和药室内壁细胞的正常代谢、绒毡层细胞的及时降解而导致花粉败育。To identify the impact of Ca2＋-ATPase on the pollen sterility process of woody plant,cerium trichloride （CeCl3） precipitation method and transmission electron microscopy were used to locate Ca2＋-ATPase in fertile and sterile anthers of Populus tomentosa Carr.The results showed that in fertile anthers,Ca2＋-ATPase distributed on the plasma membrane,the vacuole membrane and the endomembrane of the microspore mother cells （MMCs）,as well as on the plasma membrane of tapetum cells and the endomembrane of endothecium cells.Afterwards,Ca2＋-ATPase decreased in the above cells and re-accumulated at the microspores and endothecium cells at the microspore and mature pollen stage.Whereas during the development of sterile anthers,Ca2＋-ATPase was invisible on the plasma membrane of MMCs and tapetum cells at the MMC stage,while more Ca2＋-ATPases were found in the outer epidermis,endothecium and middle layer cells of sterile anthers than in fertile anthers.Finally the disintegration of tapetum in sterile anthers is not complete in sterile anthers.These results suggest that anomalous distribution of Ca2＋-ATPase in MMCs and anther walls from sterile anther may affect the active transport of calcium,probably result in abnormal calcium accumulation,and thus affect degeneration of tapetum and normal metabolism of MMCs and endothecium cells,leading to pollen abortion.

关 键 词：毛白杨 Ca2+-ATPase 花粉败育 

分 类 号：S718.46[农业科学—林学] S792.11