应用LSCM和SEM鉴定银杏花粉的倍性  

Identifying the ploidy of ginkgo pollen using laser scanning confocal and scanning electron microscope

作　　者：程金新[1,2] 孙宇涵[1] 胡君艳[1,3] 王亚茹[1,4] 丁泊元 李云[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室,林木花卉遗传育种教育部重点实验室,北京林业大学生物科学与技术学院 [2]中国人民武装警察部队学院消防工程系 [3]浙江农林大学林业与生物技术学院 [4]河北省农林科学院石家庄果树研究所

出　　处：《北京林业大学学报》2012年第4期31-34,共4页Journal of Beijing Forestry University

基　　金：高等学校博士学科点专项科研基金项目(20110014110005);国家自然科学基金项目(30771747;30471412;31170629);北京林业大学大学生科研训练计划(SRTP)(110010)

摘　　要：以经秋水仙碱诱导获得的银杏大花粉为材料,以未经处理的正常银杏花粉为对照,利用扫描电子显微镜和激光扫描共聚焦显微镜分别观察2种花粉表面形态,并分析对比其细胞内的DNA相对荧光量与银杏大花粉核内染色体倍性关系,为正确检出银杏二倍体花粉奠定重要工作基础。研究结果表明,此方法用于花粉粒的倍性鉴定科学准确,所需试验样本少,实用性强,在植物花粉倍性鉴定中有着较好的应用前景。The material used in the experiment was both the large pollen gained from the male flower buds of Ginkgo biloba,which was induced by colchicines,and the normal-sized pollen of G.biloba growing in Beijing.The ploidy of large pollen was determined by comparative surface morphology and DNA fluorescence content of pollen through the SEM and LSCM images of single cells.This method will benefit the identification of the larger pollen induced by colchicines,and it is easy to be used when the material is limited.The practicability of the method is better,therefore it has good application prospect in the ploidy identification of plant pollen from now on.

关 键 词：扫描电子显微镜 激光扫描共聚焦显微镜 银杏 花粉粒 DNA含量 倍性鉴定 

分 类 号：S722.3[农业科学—林木遗传育种]