PHGPx在体外共培养山羊睾丸生精细胞中的定位研究  

A Study on Localization of Goat PHGPx in the Co-culture Spermatogenic Cells in Vitro

作　　者：施力光[1,2] 荀文娟[2] 岳文斌[2] 张春香[2] 周汉林[1] 侯冠[1] 杨茹洁[3] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [2]山西农业大学动物科技学院,太谷030801 [3]山西农业大学太原畜牧兽医学院

出　　处：《中国草食动物科学》2012年第4期19-21,共3页China Herbivore Science

基　　金：中央级公益性科研院所基本科研业务费专项资金(1630032012022)

摘　　要：为探讨磷脂氢谷胱甘肽过氧化物酶(PHGPx)在雄性动物生殖机能中的作用及其在体外培养山羊睾丸生精细胞的定位,在已成功构建的山羊睾丸支持细胞-生精细胞共培养的基础上,制作细胞爬片,采用免疫细胞化学技术定位PHGPx的表达。结果显示,PHGPx在支持细胞、精原细胞中不表达,在圆形精子细胞细胞质检测到阳性表达产物。表明PHGPx在精子发生后期圆形精子的变态发育中起特定的调节作用,但作用机制有待深入研究。The objective of this study was to observe the expression of PHGPx in the co-culture spermatogenic cells in vitro. On the basis of the spermatogenic cells and sertoli cells co-cultured system,the slides of cells were stained for detecting the expression of PHGPx by using immunocytochemistry method. The results showed that PHGPx was mainly expressed in the cytoplasm of spermatids, but no expression in spermatogenium or sertoli cells. It suggested that PHGPx should play special roles in regulating the differentiation of spermatids during spermatogenesis, but the mechanism needed further research.

关 键 词：磷脂氢谷胱甘肽过氧化物酶 生精细胞体外共培养 山羊睾丸 细胞免疫化学 

分 类 号：S827.3[农业科学—畜牧学]