稀释液中添加谷氨酰胺对绒山羊冷冻精液品质的影响  

Effects of Glutamine with Different Levels in Diluents on Frozen Semen Quality of Cashmere Goats

作　　者：张少华[1] 任有蛇[1] 张春香[1] 李志军[1] 张建新[1] 岳文斌[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《中国草食动物科学》2012年第4期26-28,共3页China Herbivore Science

基　　金：国家科技支撑计划(2007BAD56B03)

摘　　要：在绒山羊冷冻精液稀释液中分别添加0mmol/L、2.5mmol/L、5.0mmol/L、7.5mmol/L和10.0mmol/L五个梯度的谷氨酰胺,以确定冷冻效果最好的谷氨酰胺添加浓度,优化绒山羊冷冻稀释液配方。结果表明:添加5.0mmol/L谷氨酰胺组精子的活率、顶体完整率和生存指数均极显著或显著地高于其他试验组(P<0.01,P<0.05),而畸形率极显著或显著低于其他试验组(P<0.01,P<0.05);在脂质过氧化反应和抗氧化酶活性方面,添加5.0 mmol/L组MDA浓度极显著低于其他试验组(P<0.01),而CAT活性极显著高于其他试验组(P<0.01)。总之,在稀释液中添加5.0mmol/L谷氨酰胺,在冷冻—解冻过程中能起到很好的保护精子的作用。In this experiment,for investigating the effects of glutamine on goat semen,five different levels of glutamine （0,2.5,5.0,7.5,10.0 mmol/L）were added to the diluents. The results showed that the sperm viability,acrosome integrity rate and survival index in the treatment of 5.0 mmol/L glutamine were significantly higher than those in other treatments （P〈0.01, P〈 0.05）, and the sperm abnormality was markedly lower than those in the other treatments （P〈0.01 ,P〈0.05）;the M DA content was markedly lower than those in the other treatments （P〈0.01） and the CAT activity was significantly higher than those in the other treatments （P〈0.01）. In summary, compared with other treatments, 5.0 mmol/L glutamine could provide better cryoprotec- tion for frozen-thawed cashmere goat spermatozoa than other levels.

关 键 词：稀释液 谷氨酰胺 绒山羊 冻精品质 

分 类 号：S827.3[农业科学—畜牧学]