单核细胞增生性李斯特菌分离株的生物学特性研究  

Characterization of 6 Listeria Monocytogene isolates obtained from Guangzhou poultry products

作　　者：吴晓薇[1,2] 徐成刚[1,3] 张朝冠 江经纬[1] 叶贺佳[1] 区燕宜[1] 徐小芹[1,3] 廖明[1,3] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东检验检疫技术中心,广东广州510623 [3]广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642 [4]南海出入境检验检疫局,广东佛山528200

出　　处：《中国兽医杂志》2012年第7期11-13,共3页Chinese Journal of Veterinary Medicine

基　　金：广东出入境检验检验局科技项目(2011GDK46)

摘　　要：对从广州禽产品分离的6株LM进行了药敏试验、pH值的适应范围、动物致病性等生物学特性研究。6株LM分离株对先锋必、氯霉素、卡那霉素、头孢唑啉、庆大霉素等多种药物敏感;而对多黏菌素B、复方新诺明、新生霉素有耐药性。LM分离株在pH值4.0～10.5范围内可存活。以109 CFU剂量感染SPF小鼠、清洁级新西兰兔和健康粤黄鸡,小鼠的致死率达100%,可引起兔结膜炎等症状,但未引起试验鸡的任何不适。6株LM分离株的Hly基因与标准参考株基因相似性达到96.1%以上,说明该基因在LM中较为保守。Six Listeria Monocytogene isolates were obtained from poultry products in Guangzhou. Anti-microbial resistance, suitable growth pH range and pathogenicity of the isolate were examined. All 6 isolates were sensitive to many drugs including Cefoperazone Sodium, Chloramphenicol, Kanamycin, Cefazolin V, Gentamicin; resistant to Polymyxin B, trimethoprim-sulfamethoxazole, Novobiocin. The isolates could survive in pH 4.0-10.5, and could cause BABL/c mice death with 109 CFU dose by intraperitoneal infection. The same dose of the isolates could cause conjunctivitis in New Zeeland rabbits by injection via eYe-drop route. The same dose to healthy Guangdong Huang chickens by intramuscular injection did not cause clinical signs. These hly gene nucleotide sequences had a high homology of 96.1%-100% with C53005, C53003 and DQ054589,DQ844148, EF182456 in GenBank database, so hly gene was conserved in LM.

关 键 词：单核细胞增生性李斯特菌 禽产品 生物学特性 Hly基因 

分 类 号：S852.61[农业科学—基础兽医学]