内毒素对肉鸡肠黏膜及肝线粒体复活体Ⅰ活性的影响及氨基胍的保护效应  

Effect of Endotoxemia on Activity of Complex Ⅰ in Mitochondrian of intestinal mucous membrane and liver and Protection of Aminoguanidine in Broilers

作　　者：王岩[1] 石冬梅[1] 向瑞平[1] 皇甫和平[1] 张华[1] 唐兆新[2] 徐家华[2] 王友令[2] 张胜[2] 

机构地区：[1]郑州牧业工程高等专科学校,河南郑州450011 [2]华南农业大学兽医学院,广东广州510642

出　　处：《中国兽医杂志》2012年第7期14-16,共3页Chinese Journal of Veterinary Medicine

基　　金：中国博士后科学基金(2003033411);河南省重点科技攻关(82102130014);河南省高校杰出科研人才创新工程(2004KYCX019)项目资助

摘　　要：本研究采用30日龄黄羽肉鸡90只,随机分成内毒素处理组(LPS组,5mg/kg)、氨基胍治疗组(AG+LPS组)和生理盐水对照组。每组分别在处理后1、3、5、7h和9h时间点杀鸡取样。取肠黏膜、肝组织匀浆,提取线粒体。分别检测肠黏膜和肝脏线粒体复活体Ⅰ活性。结果显示,LPS在各时间段显著降低了肉鸡肠黏膜和肝脏线粒体复合体Ⅰ的活性(P<0.05)。注射AG 1h时,肉鸡肠黏膜和肝脏线粒体复合体Ⅰ的活性未发生变化(P>0.05),3h时却显著增加(P<0.05);5h、7h时,趋于正常(P>0.05);9h时,显著降低(P<0.05)。表明LPS诱导了线粒体的损伤,引起了线粒体内的复活体Ⅰ活性的紊乱,能量代谢障碍,组织机能发生改变;AG对LPS具有明显的颉颃作用,对机体起到保护作用。A total of 90 30-day old commercial broilers were divided randomly into three groups that were respectively treated with LPS （Lipopolysaccharide from Salmonella Typhimurium, 5 mg/kg）, AG＋ LPS （Aminoguanidine, 300 mg/kg） and saline （2 mL/kg）. At different time points （1, 3, 5, 7 and 9 hour） after the injection, the mucous membrane collected, At each time point, the mitochondria of intestine, liver were taken from 6 birds per group were were determined for the evaluations of the concentrations of Complex I The results indicated that LPS significantly increased the activity of Complex I of the mitochondria in intestinal mucous membrane and liver in broilers from 1 hour to 9 hour（P〈0.05）. At 1 hour after injections with AG, the activity of Complex I did not change as compared to the control （P〈0.05）, but the activity of Complex I increased at 3 hour post injection with AG（P〈0.05）. The activity of Com- plex I did not change from 5 hour to 7 hour post injection with AG（P〈0.05）,but it significantly decreased 9 hours post AG treatment（P〈0.05）. It might be concluded that the LPS caused damages in the mitochondria, resulted in the disfunction of the energy metabolism, decreased the production of ATP energy, leading to the inhibition of the activity of Complex ~[. AG played an important role in resistance to LPS and the protection of broilers.

关 键 词：肉鸡 内毒素脂多糖、氨基胍、线粒体、复合体Ⅰ 

分 类 号：S859.796[农业科学—临床兽医学]