不同采收期栽培宽叶羌活挥发性成分的研究  

Comparison Analysis of Contents and Constituents of Volatile Oils Extracted from Cultivated Notopterygium forbesii H.Boiss Collected in Different Months

作　　者：李春丽[1,2,3] 周玉碧[2] 周国英[1,2] 徐文华[1,2] 杨路存[2,3] 

机构地区：[1]中国科学院高原生物适应与进化重点实验室,西宁810001 [2]中国科学院西北高原生物研究所,西宁810001 [3]中国科学院研究生院,北京100049

出　　处：《天然产物研究与开发》2012年第7期910-915,共6页Natural Product Research and Development

基　　金：中国科学院"西部之光"人才培养计划项目(2007年);科技部科技人员服务企业行动项目(2009GJG20016)

摘　　要：采用水蒸气蒸馏法提取不同采收时间(5、6、7、8和9月)栽培宽叶羌活药材中的挥发油,测定其含量;通过GC-MS对挥发油成分进行了分析鉴定,并采用面积归一化法计算各组分的相对含量。实验结果表明,不同采收时间,栽培宽叶羌活挥发油含量存在差异,以8月份采收的药材挥发油含量最高;挥发油经GC-MS分析,共鉴定出39个化合物,有31种共有成分;对共有组分进行主成分分析显示,香桧烯、α-蒎烯、莰烯、β-蒎烯、γ-萜品烯、乙酸龙脑酯、α-红没药醇等15种成分可作为挥发油季节变化的特征组分。不同季节采集的羌活生药材,其挥发油含量和成分具有一定的差别,在一定程度上反映了其药用价值的微妙差异,可为羌活药材药理药用价值的进一步开发利用提供一定的参考。To determine the contents and constituents of volatile oils extracted from cultivated Notopterygium forbesii H.Boiss vegetated in different months.Analyzed their seasonal dynamics and provided experiment foundation for reasonable utilization of Rhizoma et Radix Notopterygii.Volatile oils were extracted by steam distillation.The chemical constituents were separated and identified by gas chromatography-mass spectrometer（GC-MS）.The relative content of each component was determined by area normalization.The difference of volatile oil contents was significant at the 0.01 level between different samples.The content was highest in the sample collected in August,and lowest in the sample vegetated in May.Thirty nine constituents were identified by GC-MS analysis,and there were thirty one same compounds in the five samples.The principal components analysis of SPSS was applied to the study of characteristic compounds and separated 15 kinds of compounds as characteristic constituents of volatile oil extracted from cultivated N.forbesii H.Boiss.vegetated in different months.The contents and constituents of volatile oils had obvious seasonal dynamics in the cultivated N.forbesii H.Boiss.To a certain extent,these reflected the subtle difference of its medical value.

关 键 词：宽叶羌活 挥发油 组分分析 季节动态 

分 类 号：R284.2[医药卫生—中药学] Q946.91[医药卫生—中医学]