改构型酸性成纤维细胞生长因子对白花丹素诱导的人胚肝细胞L-O2损伤的保护作用  

The Protective Effect of aFGF on Human Liver L-O2 Damaged by Plumbagin in vitro

作　　者：韦敏[1] 刘华钢[2] 李丹妮[2] 

机构地区：[1]广西壮族自治区柳州市人民医院,545006 [2]广西壮族自治区药监局,广西南宁530000

出　　处：《时珍国医国药》2012年第7期1617-1619,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30850010)

摘　　要：目的研究改构型酸性成纤维细胞生长因子(aFGF)对白花丹素引起的人胚肝细胞L-O2损伤的保护作用及机理。方法用白花丹素体外诱导肝细胞损伤,同时用aFGF对肝细胞进行保护,测定培养上清液中的SOD,MDA,LDH值,MTT法测定肝细胞活性。结果与损伤组比较,aFGF对白花丹素引起的胚肝细胞损伤有明显的保护作用(P<0.05)。结论 aFGF可保护肝细胞,其机制可能与其拮抗自由基产生有关。Objective To study the protective effect and possible mechanism of aFGF on human liver cell L-O2 injured by plumbagin in vitro. Methods Human liver cell L-O2 damaged by plumbagin.aFGF were added to cultural solution of human liver cell L-O2.The contents of SOD and MDA and activity of LDH in cultural supermatant were determined by general methods,the viabilities of hepatocytes were measured by MTT. Results Compared with model group,aFGF could protect human liver cell L-O2 from damage of plumbagin（P〈0.05）. Conclusion aFGF has a protective action on human liver cell L-O2 from being damaged by plumbagin.

关 键 词：AFGF 人胚肝细胞L-O2 白花丹素 保护作用 

分 类 号：R962[医药卫生—药理学]