仙人掌多糖主要组分成分分析及其对体外培养免疫细胞的影响  

Composition Analysis of the Main Components of Opuntia dillenii Haw. Polysaccharides and Effects of ODP-I on Immune Cell in vitro

作　　者：赵龙岩[1,2] 张松莲[1,2] 袁清霞[1,2] 程杰[1,2] 陈晓慧[1] 吴艺华[1] 曾富华[1,2] 

机构地区：[1]湛江师范学院生命科学与技术学院,广东湛江524048 [2]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《时珍国医国药》2012年第7期1653-1656,共4页Lishizhen Medicine and Materia Medica Research

基　　金：广东省自然科学基金(No.8152404801000011);广东省湛江市科技计划项目(No.2011D032)

摘　　要：目的分析野生仙人掌多糖(ODPs)主要组分单糖组成,探讨ODPs主要组分ODP-I的免疫活性,为ODPs构效关系及产业化提供参考。方法采用凝胶层析方法,分离纯化得到ODPs各组分,用优化的苯酚-硫酸法测定ODPs纯度;用HPLC-ELSD和硫酸-咔唑法分析ODPs单糖组成;并测定ODPs中蛋白质含量。结果 ODPs各组分纯度平均达90%以上,几乎不含蛋白质,主要由4种单糖组成,糖醛酸含量较高;ODP-I可极显著地促进小鼠脾淋巴细胞自然增殖;促进T和B淋巴细胞的增殖,且呈剂量依赖关系;促进巨噬细胞的吞噬功能、代谢活性及分泌NO的能力。结论 ODPs为含有至少4种单糖的杂多糖,ODP-I能从免疫系统的多个方面来提高机体的免疫力,具有良好的免疫活性。Objective To analyse the monosaccharide composition of Opuntia dillenii Haw.polysaccharides（ODPs） components,to determine effects of their main component on the immune function of mice and to provide the reference for the structure-activity relationship and industrialization of ODPs. Methods The components of ODPs were isolated by Gel chromatography and the contents of ODPs were determined by the optimized phenol-sulfuric acid method.The monosaccharide composition of ODPs were analysed by HPLC-ELSD and sulfuric acid-carbazole method.The contents of protein in ODPs were determined by Coomassie brilliant blue method. Results The average polysaccharide content of the main components of ODPs was above 90%,and they did not contain protein nearly.They were composed of 4 kinds of monosaccharides and their contents of uronic acid were high.ODP-I significantly stimulated the proliferation of spenocytes,T and B lymphocytes,which all presented dose-effect relationship.ODP-I also significantly enhanced the function of macrophage,the metabolic activity and NO production of macrophage in mice. Conclusion ODPs are heteropolysaccharides which contain at least 4 kinds of monosaccharides.ODP-I has good immune activity and can enhance immunity through many aspects.

关 键 词：野生仙人掌 多糖 成分分析 免疫 

分 类 号：R285.5[医药卫生—中药学]