三角草中三角草苷A的含量测定(英文)  

The Content of Chlorophytoside A in Chlorophytum laxum

作　　者：吴惠妃[1] 梅全喜[1] 高幼衡[2] 王岩[3] 张东京[3] 

机构地区：[1]广州中医药大学附属中山医院,广东中山528400 [2]广州中医药大学,广东广州510006 [3]广东药学院,广东广州510006

出　　处：《时珍国医国药》2012年第7期1788-1790,共3页Lishizhen Medicine and Materia Medica Research

基　　金：Medical science foundation of the administration of TCM of Guangdorg Province(Grant No.101127)~~

摘　　要：目的建立三角草中三角草苷A的含量测定方法。方法高效液相色谱法测定三角草苷A的含量,采用Agilent TC-C18(Z)色谱柱(250 mm×4.6 mm,10μm),紫外检测波长为200 nm,以乙腈-1%磷酸溶液(24∶76)为流动相,流速为1.0 ml.min-1,柱温为25℃,进样量10μl,按外标法进行检测。结果供试品中三角草苷A得到较好的分离与测定,三角草苷A在0.48～4.8μg范围内线性关系良好,回归方程为:A=1E+06x-28 019,r2=0.999 9,平均回收率达99.02%,RSD=0.81%(n=6)。结论所建立的方法快速,简便,准确,重复性好,专属性好,可用于评价三角草的质量。Objective To establish an HPLC method for the determination of Chlorophytoside A in Chlorophytum laxum R.Br. Methods A high performance liquid chromatography was used.The measurement conditions were as follows： TC-C18（Z） Agilent column（250mm×4.6mm,10μm）;mobile phase： acetonitrile and 1% phosphoric acid（24：76）;detection wavelength： 200nm;column temperature： 25℃;flow rate： 1.0 ml/min;injection：10μl. Results Chlorophytoside A was separated well on the column from other components of the sample,the linear range of chlorphenamine maleate was 0.48μg～4.8μg,the standard curve of chlorphenamine maleate was as follows： y =1E＋06x-28019,r2=0.9999,the average recovery of Chlorophytoside A was 99.02% with RSD 0.81%（n=6）. Conclusion The method is rapid,simple,accurate,reproducible and selective,which can be used for the quality control of Chlorophytum laxum.

关 键 词：高效液相色谱法 三角草 三角草苷A 含量测定 

分 类 号：R284.2[医药卫生—中药学]