内标校正法在栀子药材多成分定量分析中的应用  

Application of Internal Standard Correction Method in Multi-component Quantitative Analysis of Fructus Gardeniae

作　　者：娄素卉[1,2] 谢媛媛[2] 王义明[2] 梁琼麟[2] 罗国安[1,2] 

机构地区：[1]华东理工大学药学院,上海200237 [2]清华大学,北京100084

出　　处：《时珍国医国药》2012年第7期1812-1814,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家"十一五""重大新药创制"科技重大专项(No.2009ZX09308-004)

摘　　要：目的尝试内标校正法在栀子不同类化合物定量分析中的应用。方法利用高效液相色谱质谱联用技术(HPLC/DAD-MS)对栀子中京尼平龙胆二糖苷、栀子苷、绿原酸、西红花苷I和西红花苷II进行定性定量分析。采用内标校正法,以栀子苷或西红花苷I为参照物,考察其他成分相对参照物的校正因子(fkm),进行系统适用性考察,并比较计算值与实测值的差异。结果分别以栀子苷或以西红花苷I为参照物,评价栀子中栀子苷、西红花苷I和西红花苷II的含量是可行的,校正因子系统适用性良好(RSD<5%),内标校正法测定结果与实测值基本一致(RSD<5%)。结论内标校正法实用、高效,可用于特定不同类成分含量测定。Objective Internal standard correction method（ISCM） was applied to quality evaluation of different kind compounds in Fructus Gardeniae（FG）. Methods Qualitative and quantitative analysis of Genipin-1-gentiobioside,Geniposide,Chlorogenic acid,Crocin I and Crocin II in FG were firstly made by HPLC/DAD-MS.Geniposide and Crocin I were taken as references separately to determine the other four components.Correction factors（fkm） were calculated,and system suitability was studied. Results Geniposide and crocinI could be taken as references to determine Geniposide,Crocin I and Crocin II.The system suitability was good（RSD〈5%） and results of ISCM and standard curve method was basically the same（RSD〈5%）. Conclusion ISCM is economical,practical and efficient,and can be used for quantitative analysis of certain different kind components.

关 键 词：栀子 内标校正法 高效液相色谱质谱联用 

分 类 号：R284.2[医药卫生—中药学]