陆地棉矮化突变体Ari1327的矮化机理研究  

Study on the Dwarf Mechanism of an Upland Cotton(G.hirsutum L.) Dwarf Mutant,Ari1327

作　　者：王新坤[1,2] 孙君灵[1] 潘兆娥[1] 张超[3] 何守朴[1] 贾银华[1] 周忠丽[1] 唐灿明[2] 杜雄明[1] 

机构地区：[1]中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南安阳455000 [2]南京农业大学,江苏南京210095 [3]四川省农业科学院,四川简阳641400

出　　处：《棉花学报》2012年第4期293-302,共10页Cotton Science

基　　金：国家重点基础研究发展计划(973计划)项目(2004CB117306)

摘　　要：Ari1327是从美国引进的陆地棉种质Ari971经60Coγ射线照射后得到的一个新型矮化突变体。Ari1327在萌发期和子叶期就表现出矮化性状。在现蕾期,突变体的株高、节间数和节间平均长度均小于野生型,差异达到极显著水平,节间平均长度的缩短导致了突变体株高的降低。突变体主茎节间纵向细胞的长度要显著小于野生型,横向细胞的直径与野生型差异不显著,节间细胞伸长受抑制导致了突变体节间平均长度的缩短。突变体中IAA、GA3和ABA含量高于野生型,外施GA3能使其株高恢复至野生型水平,外施BR和IAA不能使其株高恢复到正常水平,较低浓度的IAA对突变体的株高和节间长度就开始产生抑制作用。突变可能导致了IAA合成相关基因的过量表达,突变体内源IAA含量急剧升高而抑制了植株生长,使株高降低。Plant height is an important agronomic trait in upland cotton. Ari1327 is a dwarf mutant derived from an American upland cotton variety, Ari971, by 60Co γ-ray irradiation. Ari1327 exhibits the dwarf trait during the germination and cotyledon periods. During the budding period, the plant height, internode number and intemode length of Aril327 were significantly（P〈 0.01） smaller or fewer than in the wild type. Therefore, the mutant dwarfism was due to the decrease of average intemode length. The longitudinal cell length of mutant intemodes was significantly shorter than in the wild type（P 〈 0.01）. The difference in transverse cell diameter between the mutant and the wild type was not significant. Thus, the shorter internodes were induced by inhibition of internode cells. The IAA, GA3 and ABA content of Ari1327 was higher than in the wild type. The height of Ari1327 plants could be restored to wild type levels by exogenous GA3 application. Exogenous BR and IAA had no effect on Ari 1327. Due to over-expression of genes in the IAA synthetic pathway, the IAA content in Ari1327 was remarkably increased. High-density IAA inhibits plant growth; thus, the plant exhibits a dwarfphenotype.

关 键 词：棉花(G.hirsutumL.) 矮化 机理 

分 类 号：S562[农业科学—作物学]