β-环糊精衍生物流动相添加剂应用于HPCE中分离布洛芬  

Separation of Ibuprofen in HPCE with β-Cyclodextrin Derivative

作　　者：沈静茹[1] 朱财延[1] 余学红[2] 韦慧慰[1] 邱思杨[1] 

机构地区：[1]中南民族大学化学与材料科学学院分析化学国家民委重点实验室,武汉430074 [2]中南民族大学校医院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2012年第2期10-14,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(95J60)

摘　　要：以双-[-6-氧-(-2-间羧基苯磺酰基-丁二酸-1,4-单酯-4-)-]-β-环糊精(β-CD-B2)为手性添加剂在高效毛细管电泳(HPCE)中分离布洛芬,考察了缓冲溶液硼砂的浓度、pH值,手性添加剂β-CD-B2的浓度,分离电压对布洛芬手性异构体分离的影响.结果8min内实现了对映体的基线分离,最佳分离条件下分离度Rs为4.26.布洛芬对映体分离的前峰线性范围为0.0538～0.942g/L,后峰线性范围为0.178～0.942g/L.在分离布洛芬的最佳条件下,可同时分离布比卡因和布洛芬2种手性药物的混合溶液.Chiral separation of ibuprofen was investigated by bis- L -6-oxygen - （ -2-m-carboxyl phenylsulfonyl - succinic acid 1,4 - ester -4 -）-]-β-cyclodextrin （β-CD-B2 ） as chiral additive in high performance capillary electrophoresis （HPCE）. The effect of concentration and pH of borax buffer solution, concentration of chiral additive β-CD-B2 and separation vohage on the separation were discussed. The results indicated that the HPCE resolution （ RS ） under optimum condition was 4.26 in 8 min . Linear range of ibuprofen enantiomers＇ s separated front peaks is 0. 053 - 0. 942g/L, while later peak linear range is 0. 178 -0.942g/L. Thus ibuprofen and bupivacaine, two chiral drugs can be separated under the same condition best suited for ibuprofen.

关 键 词：高效毛细管电泳 Β-环糊精衍生物 流动相添加剂 手性分离 布洛芬 

分 类 号：O657.8[理学—分析化学]