26-失碳-8-氧代-α-芒柄花萜醇对体外培养成骨细胞活性的影响  

Effects of 26-NO-Ono on Activity of Cultured Osteoblasts in vitro

作　　者：王朝元[1] 宋超[1] 唐俊龙[1] 魏甜甜[1] 易继凌[1] 杨光忠[2] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2012年第2期33-37,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(2009CDZ033)

摘　　要：为研究玉柏石松提取物26-失碳-8-氧代-α-芒柄花萜醇(26-NO-Ono)对成骨细胞活性的影响,采用MTT检测不同浓度26-NO-Ono(3.33,6.66,13.32,26.64μmol/L)对成骨细胞增殖率,碱性磷酸酶(ALP)试剂盒检测成骨细胞内ALP活性,荧光定量PCR检测成骨细胞骨相关基因表达.结果表明:26-NO-Ono给药1d可促进成骨细胞增殖,给药3d可促进成骨细胞ALP活性.26-NO-Ono处理3d和9d会抑制骨涎蛋白(BSP)、I型胶原蛋白(Col-I)以及骨钙素蛋白(OCN)的基因表达;处理6d会促进上述基因的表达.26-NO-Ono长期处理(6d和9d)可以抑制骨桥蛋白(OPN)的基因表达,说明26-NO-Ono对成骨细胞的成骨活性的影响呈时间依赖性,剂量依赖性和细胞分化状态依赖性.To study the effects of 26-nor-8-oxo-α-onocerin （26-NO-Ono）, an extract from Lycopodium obscurum L. , on the activity of cultured osteoblasts in vitro, different concentrations （3.33, 6.66, 13.32, 26. 641μmol/L） of 26-NO-Ono were given to cultured osteblasts. MTF assay, alkaline phosphate （ALP） kit, real-time PCR were applied to measure the proliferation rate, activity of ALP and expression of bone-related genes in treated osteoblasts. Results showed that treatment with 26-NO-Ono for 1 d can promote the osteoblast proliferation rate and 3 d can enhance the ALP activity. Treatment with 26-NO-Ono for 3 d and 9 d can inhibit the expression of bone-related genes such as BSP, Col- I and OCN in osteoblasts, while treatment for 6 d can stimulate the above 3 genes. Meanwhile, treatment with 26-NO-Ono for 6 d and 9 d could inhibit the expression of OPN gene. Our results indicated that the effect of 26-NO-Ono on osteogenic activity of osteoblast is time-dependent, concentration-dependent and differentiation state-dependent.

关 键 词：26-失碳-8-氧代-α-芒柄花萜醇 成骨细胞 细胞增殖 碱性磷酸酶活性 骨相关基因mRNA的表达 

分 类 号：Q813.1[生物学—生物工程] Q786