不同民族大学生乙肝病毒基因型分布状况调查  

Study on the Distribution of HBV Genotype among Undergraduates from Different Nationalities

作　　者：李劲[1] 刘莹[1] 邓志芳[1] 胡文娟[1] 高奎[1] 赵瑞杰[1] 王红[2] 宋发军[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]中南民族大学校医院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2012年第2期38-41,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家民委自然科学基金资助项目(07ZN09/MZY07004)

摘　　要：为了解不同民族大学生乙型肝炎病毒(hepatitis B virus,HBV)基因型和亚型分布状况,采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术,对中南民族大学93例来自15个民族乙肝大三阳学生血清HBV进行基因分型.结果表明:患者中HBV基因B型占61.3%(57/93),C型占25.8%(24/93),D型占4.3%(4/93).说明各民族HBV基因型分布中,B型占绝对优势,C型次之,D型最少(P<0.01).且不同民族不影响HBV基因型的分布(P>0.05),C型和D型分布基本符合我国HBV基因型地理区域分布规律.To investigate the distribution of Hepatitis B virus genotype and subgenotype among undergraduates from various nationalities, 93 HBV-DNA positive serum samples from 15 nationalities in South-Central University for Nationalities were analyzed by PCR-RFLP. The results indicated that HBV genotype B accounted for 61.3% （57/93）, genotype C accounted for 25.8% （24/93） and genotype D accounted for 4.3% （4/93）. This demonstrated that genotype B is dominant in all the nationalities, genotype C ranks secondly and least genotype D （ P 〈 0. O1 ）. Ethnic differences have no effect on the classification of HBV genotype （ P 〉 0. 05 ）. Moreover, distribution of genotype C and D basically coincides with the geographic distribution of HBV in China.

关 键 词：乙型肝炎病毒 A-H基因型分布 限制性片段长度多态性聚合酶链反应 

分 类 号：R373.21[医药卫生—病原生物学] R181.26[医药卫生—基础医学]