梅花鹿鹿茸总蛋白提取方法对双向电泳图谱的影响  

Methods of Antler Total Protein Extraction on the Effect of Map Quality of Two-dimensional Electrophoresis in Sika Deer

作　　者：赵东[1,2] 邢秀梅[2] 杨福合[2] 

机构地区：[1]江苏科技大学生物与化学工程学院,江苏镇江212018 [2]中国农业科学院特产研究所,吉林省特种经济动物分子生物学国家重点实验室,农业部特种经济动物遗传育种与繁殖重点实验室,吉林省特种经济动物分子生物学重点实验室,吉林吉林132109

出　　处：《中国畜牧兽医》2012年第7期25-28,共4页China Animal Husbandry & Veterinary Medicine

基　　金：中国农业科学院院长基金资助(采用蛋白质组学方法鉴别不同发育阶段鹿茸蛋白(201004))

摘　　要：鹿茸是唯一可以重复再生的哺乳动物器官。利用不同的提取方法制备梅花鹿鹿茸总蛋白样品,分别进行双向电泳,从得到的蛋白点的数量和点的圆滑程度、分辨率等确定适合的提取方法。分别用全蛋白提取试剂盒、自制裂解液、丙酮沉淀制备蛋白样品,并对得到的3种2-DE图谱进行比较分析。结果显示,试剂盒提取法和自制裂解液法得到的蛋白点比较多、圆滑,图谱清晰;丙酮沉淀法得到的蛋白点不如前者多,图谱有横竖纹。Deer antlers are the only mammalian organs capable of repeated regeneration. To prepare total protein samples of sika deer antler for 2 DE by different extraction methods, and then find a suitable one from the spots number and resolution condition in the map. Total protein extraction kit, manual lysate and acetone precipitation were used and three kinds of 2-DE maps were analyzed. The results showed that spots number and resolution of kit and manual lysate were better than that of ac- etone precipitation, and stripes in the map of acetone precipitation were visible.

关 键 词：鹿茸 蛋白样品 提取方法 双向电泳 

分 类 号：Q51[生物学—生物化学]