变性梯度凝胶电泳技术及其在动物肠道菌群研究中的应用  

Review on Denaturing Gradient Gel Electrophoresis and its Application in Animal Intestinal Bacterial Community

作　　者：赵春苗[1] 徐春厚[1] 

机构地区：[1]广东海洋大学动物医学系,广东湛江524088

出　　处：《中国畜牧兽医》2012年第7期62-65,共4页China Animal Husbandry & Veterinary Medicine

基　　金：广东省科技计划项目(2008B020800007)

摘　　要：变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术是一种新型的用于检测核酸变异和点突变的电泳方法,主要根据突变型和野生型核酸序列的不同而导致其变性浓度的差异,从而利用变性梯度胶进行分离,可用于肠道菌群结构多样性和种群动态变化的研究。文章对DGGE技术的原理、在肠道菌群多样性和动态性的应用、局限性及发展前景及上综述。Denaturing gradient gel electrophoresis （DDGE） was a new electrophoresis method for testing the nucleic acid variation and point mutations, mainly based on the different denaturing concentrations dued to the different nucleic acid se quence of mutant type and wild type, thus using the denaturing gradient glue to make it separate. It could be used in the diver- sity and dynamics analyses of microbial communities. The principles of DGGE, applications in diversity and dynamics of intes- tinal bacterial community, the limitations and future developing perspectives were summarized in this paper.

关 键 词：变性梯度凝胶电泳 肠道菌群 多样性 动态变化 

分 类 号：Q503[生物学—生物化学]