伊维菌素小单室脂质体工艺配方优化及质量分析  

Optimizing Process and Formula and Analyzing Quality for Ivermectin Single Unilamellar Vesicles

作　　者：何书海[1] 巴桑旺堆[2] 佘永新[3] 

机构地区：[1]信阳农业高等专科学校动物科学系,河南信阳464000 [2]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850009 [3]中国农业科学院农业质量标准与检测技术研究所,北京100081

出　　处：《中国畜牧兽医》2012年第7期108-112,共5页China Animal Husbandry & Veterinary Medicine

基　　金：西藏自治区科技厅重点项目(NO10-1)

摘　　要：为获得高包封率的伊维菌素小单室脂质体,本研究对其制备工艺进行了优化,并选取超声波频率、超声时间、卵磷脂与胆固醇质量比、伊维菌素与卵磷脂质量比各因素进行正交交互作用考察,同时利用反相高效液相色谱法对各工艺配方组合下伊维菌素小单室脂质体的包封率进行了测定。结果显示,伊维菌素小单室脂质体的制备最佳配方和工艺组合为超声波频率200kHz,超声时间6min,伊维菌素与大豆卵磷脂质量比为1∶10。经过优化组合,得到了较高品质的伊维菌素小单室脂质体,平均载药量达到(92.35±0.61)%,药物品质优良,且制备工艺简单可行。To obtain high entrapment rate of ivermectin single unilamellar vesicles. The study was aim to optimizing prepa ration process of ivermectin single unilamellar vesicles; we had selected some factors such as ultrasonic frequency, ultrasonic time, mass ratio of lecithin to cholesterol and mass ratio of ivermectin to lecithin to design orthogonal test L8 （2^7）, and we used RP-HPLC to determine the entrapment rate of ivermectin single unilamellar vesicles under every combination of process and formula. The results showed the optimal process and formula combination of ivermectin single unilamellar vesicles were as fol- lows： the ultrasonic frequency was 200 kHz, the ultrasonic time was 6 minutes and the mass ratio of ivermectin to lecithin was 1 ： 10. We have obtained high qualitative ivermeetin single unilamellar vesicles by optimization,the loading rate reach （92.35 0.61） %, the quality of drug is fine, the preparation process are simple and feasible.

关 键 词：伊维菌素 小单室脂质体 制剂技术 质量分析 正交试验 

分 类 号：S859.795[农业科学—临床兽医学]