犊牛超数排卵方法的研究  

Study on the Method of Superovulation in Calves

作　　者：郑自华[1] 毛献宝[1] 陈宝剑[1] 潘斌[1] 黎江[1] 范晓明[1] 沈创[2] 韦英明[1,2] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005 [2]广西大学"863"计划南方良种牛繁育中心,广西南宁530005

出　　处：《中国畜牧兽医》2012年第7期183-188,共6页China Animal Husbandry & Veterinary Medicine

基　　金：广西自然科学基金项目(桂科自0832037)

摘　　要：本试验采用不同超排处理方案对不同月龄犊牛(1～6月龄)进行超排处理,以得出犊牛超数排卵的最佳方案、最佳月龄和重复超排的最佳时间间隔。结果表明,超排方案4的超排效果最好,平均采卵数(80.0枚)、平均回收卵数(43.3枚)和平均可用卵数(37.8枚)均显著高于其他4个方案(P<0.05),表明超排方案4比较适合3月龄犊牛的超数排卵;2、3、4、5月龄犊牛应用超排方案4的超排效果较好,差异不显著,平均可用卵数分别为33.5、41.0、34.5、32.8枚,1、6月龄犊牛的超排效果显著差于其他4个月龄组(P<0.05),平均可用卵数分别为10.8、18.3枚,表明超排方案4处理犊牛的最佳月龄为2～5月龄;超排间隔2周组(6.3枚)平均可用卵数效果显著差于间隔3、4和5周组(16.7、23.7、23.0枚)(P<0.05),超排间隔3、4、5周组之间超排效果差异不显著,表明犊牛重复超排处理的最佳时间间隔为3～4周。In order to define the best superovulation program, the best month old of calves and the best repeated superovu- lation time interval, different superovulation programs were conducted on different months old calves （1-6 months old） in this study. The results showed that the superovulation program 4, of which the average number of eggs collected was 80.0 mis- siles, the average number of oocytes recovered was 43.3 missiles and the average number of eggs available was 37.8 missiles, were significantly higher than the other four programs （P〈0.05）. It suggested that program 4 was fitted for superovulation of 3 months old calves; program 4 was much better than other 4 programs in 2 months old, 3 months old, 4 months old, 5 month old calves with the average number of eggs of 33.5, 41.0, 34.5 and 32.8 missiles respectively, and the difference between each other was not significant. The results of 1 month and 6 months old calves with the average number of available eggs of 10.8 and 18.3 missiles was significantly lower than the other four age groups （P%0.05）, it suggested that the superovulation treat ment program 4 was most suitable for 2 to 5 months calves. Moreover, we also found that the results of superovulation interval of 2 weeks group with average number of eggs available of 6.3 missiles were significantly worse than the interval of 3 weeks, 4 weeks and 5 weeks group （P〈0.05） with that of 16.7, 23.7, 23.0 missiles, respectively. The superovulation difference be- tween 3 weeks, 4 weeks, 5 weeks groups was not significant indicated that the best time interval of 3 to 4 weeks was best for repeated superovulation treatment on calves.

关 键 词：犊牛 超数排卵 超排方案 

分 类 号：S814.4[农业科学—畜牧学]