一株猪源屎肠球菌的分离、鉴定及益生功能初步研究  

Isolation,Identification of Enterococcus faecium Strain and Preliminary Study on its Beneficial Effects

作　　者：王凡[1] 王建设[1] 刘婷[1] 李晓清[1] 王安如[1] 

机构地区：[1]北京大北农科技集团股份有限公司,饲用微生物工程国家重点实验室,北京100193

出　　处：《中国畜牧兽医》2012年第7期231-234,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家973计划项目(2010CB35703);北京市海淀区重大科技研发项目(K20100130Z);北京市2010年度促进科技成果转化落地区县项目(Z101105053710013)

摘　　要：从健康仔猪粪便中分离到一株乳酸菌ZW002,并从形态、生理生化指标,16SrRNA基因序列分析,鉴定该菌株为屎肠球菌(Enterococcusf aecium),将其16SrRNA基因提交GenBank,登录号为JN542513。从耐酸、耐胆盐及抑菌性能等方面对菌株进行益生功能初步研究,结果表明,菌株ZW002表现出良好的耐受性及益菌性能,为猪用微生态制剂的研究开发奠定了基础。In this article,strain ZW002 belonged to Lactobacillus was isolated from healthy pig feces. According to the re- sults of morphological, physiological, biochemical tests,and sequence analysis of 16S rRNA gene, strain ZW002 was identified as Enterococcus faecium,the accession number of 16S rRNA gene is JN542513. Moreover, the preliminary study of its beneficial effects,like acid resistance,hile salt tolerance and antibacterial activity was carried out. The results showed that strain ZW002 exhibited good stress tolerance and antibacterial activity,which lead strain ZW002 to be a promising candidate for probiotic re- search.

关 键 词：益生菌 屎肠球菌 鉴定 益生性能 

分 类 号：Q78[生物学—分子生物学]