鸡毒支原体穿梭质粒的构建  

Construction of shuttle vector for Mycoplasma gallisepticum

作　　者：胡美容[1,2] 陈鸿军[2,3] 于圣青[2,3] 谭磊[2,3] 仇旭升[2,3] 高崧[1] 丁铲[2,3] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]中国农业科学院上海兽医研究所国家家禽工程技术研究中心,上海200241 [3]国家家禽工程技术研究中心,上海200331

出　　处：《中国预防兽医学报》2012年第8期619-623,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30871883;31001077)

摘　　要：为构建大肠菌(E.coli)-鸡支原体(MG)穿梭质粒,本研究基于MG复制原点(oriC)部分活性序列区域,以pBluescript-SK(+)为骨架载体,庆大霉素为筛选基因,构建了E.coli-MG穿梭质粒pBSoriC-GmR,经PCR及western blot检测该载体能够在MG中稳定存在22代以上,并能够高效表达庆大霉素乙酰转移酶。本研究为利用该载体进行MG基因功能的研究奠定了基础。To construct a shuttle vector for Mycoplasma gallisepticum, the shuttle plasmid of pBSoriC-GmR was generated by insertion of a partial oriC region of M. gallisepticum into pBluescript-SK（＋） vector with the gentamicin as the antibiotic gene. The results of PCR and western blot showed that pBSoriC-GmR shuttle vector was able to exist stably and highly expressed gentamicin acetyltransferase up to 22 passages in M. gallisepticum. The construction of shuttle vector would facilitate for further study of M. gallisepticum.

关 键 词：鸡毒支原体 复制原点 ORIC 可复制载体 

分 类 号：S852.62[农业科学—基础兽医学]