布鲁氏菌弱毒疫苗粘膜免疫及检测方法的研究  

Eye drop vaccination against sheep brucellosis and tests for distinguishing between vaccinated and nature infected sheep

作　　者：赵兵[1] 彭传真 张志新 赵天博 陈荣贵 王海军 马文勇 努尔.赛力克 伊恒博 曾凡明 任明 何倩倪[1] 

机构地区：[1]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000 [2]哈密地区动物疫病诊断控制中心,新疆哈密839001 [3]新疆警官高等学院,新疆乌鲁木齐830011 [4]新疆伊犁动物疫病诊断控制中心,新疆伊宁835000 [5]新疆新源县动物疾病控制中心,新疆新源835800 [6]新疆尼勒克县动物疾病控制中心,新疆尼勒克835700

出　　处：《中国预防兽医学报》2012年第8期658-661,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家农业公益行业科研专项(200803026)

摘　　要：为研究布鲁氏菌弱毒疫苗粘膜免疫及其检测方法,本实验采用粘膜点眼途径对健康母羊接种布鲁氏杆菌猪2号疫苗(S2)、牛19号疫苗(A19)和羊强毒株(M16),筛选布鲁氏杆菌病鉴别检测方法。将12月龄～14月龄母羊60只随机分为3组,以常规疫苗推荐剂量进行半量粘膜点眼接种。采集血液、淋巴、脏器进行布鲁氏菌病血清学检测和细菌学分离以及PCR检测。结果表明:布鲁氏菌弱毒疫苗抗体水平持续6个月,其中血清学的试管凝集试验、半胱氨酸凝集试验与补体结合试验的阳性符合率达到100%。细菌分离期为6个月,乳腺、乳腺淋巴、髂淋巴分离率较高;而强毒株M16的抗体水平和细菌分离持续12个月以上。结果显示以常规血清学和细菌学检测方法在点眼免疫布鲁氏菌S2、A19苗6个月后可以进行野毒感染和疫苗免疫畜的鉴别诊断。To differential detection of brucellosis in vaccinated and nature infected sheep, three groups of ewes aged 12 months to 14 months （20 for each group） were divided including two groups vaccinated via eye drop with live attenuated vaccine Brucella S2 （Brucella swine） and A19 （B. abortus） respectively with half dose of vaccination, and one group infected with a pathogenicity strain M16 （B. melitensis） to determine the sensitivity of serum agglutination test （SAT）, the cysteine agglutination test （CAT） and the complement fixation test （CFT）） for distinguishing vaccinated and infected sheep. The results showed that specific antibodies against the vaccines were lasted at detectable levels for 6 months post vaccination. But the virulent strain of M16 was 100% detectable after 12 months of infection, the bacterial isolates were existed at high titres in breast cells, breast lymph nodes and iliac lymph. We conclude that the conventional serological and bacteria isolation methods could be used for distinguishing between vaccinated and infected sheep after 6 months of vaccination and infection.

关 键 词：布鲁氏菌苗 粘膜免疫 安全性 可鉴别诊断性 

分 类 号：S852.61[农业科学—基础兽医学]