二氮嗪预处理对大鼠部分肝移植的保护作用  

Protective Effect of Diazoxide Preconditioning in Partial Liver Transplantation in Rats

作　　者：宋康[1] 孙绮蛮[1] 周俭[1] 黄晓武[1] 史颖弘[1] 肖永胜[1] 谭长军[1] 高强[1] 何义舟[1] 成剑文[1] 杨柳晓[1] 樊嘉[1] 

机构地区：[1]复旦大学附属中山医院肝外科,上海200032

出　　处：《中国临床医学》2012年第3期222-224,共3页Chinese Journal of Clinical Medicine

基　　金：复旦大学青年骨干科研启动基金(编号:B-195)

摘　　要：目的:探讨二氮嗪(diazoxide,DA)预处理对大鼠部分肝移植的保护作用,寻找减轻临床部分肝移植中缺血再灌注损伤的途径。方法:将150只SD(Sprague-Dawley)大鼠,随机配对成75对,分为3组:对照组(C组)、DA预处理组(DA组)和5-羟基癸酸盐(5-hydroxydecanoate sodium salt,5-HD)+DA预处理组(HD-DA组),每组25对。在进行60%比例部分肝移植术前,C组供体不进行药物预处理;DA组供体在术前30min腹腔注射DA(5mg/kg);HD-DA组供体在术前45min腹腔注射5-HD(5mg/kg),并在其后15min腹腔注射DA(5mg/kg)。各组分别在术后2h、1d、3d、5d和7d各处死5只受体大鼠,测定谷酸丙氨酸转移酶(alanine aminotransferase,ALT)和门冬氨酸氨基转移酶(aspartate aminotransferase,AST),并在光镜下进行病理检查,电镜下进行超微结构检查。结果:DA组与C组相比,肝细胞肿胀、变性、坏死和空泡样变性以及肝小叶结构排列紊乱和汇管区炎症都较轻,而HD-DA组则跟与C组相近。DA组术后2h、1d、3d的ALT值和AST值均显著低于C组(P<0.05),而HD-DA组与C组相比,各时点ALT和AST值均无显著差异(P>0.05)。结论:二氮嗪预处理对大鼠部分肝移植具有保护作用,但这种保护作用可以被5-羟基癸酸盐特异性阻断。t Objective：To investigate the protective effect of diazoxide preconditioning in partial liver transplantation in rats. Methods：Seventy five pairs of Sprague-Dawley rats were randomly divided into 3 groups and each group consisted of 25 pairs. Rats in all the 3 groups underwent partial liver transplantation. Donor rats in one group （Group DA） were treated by intrape- ritoneal injection of diazoxide （5 mg / kg） 30 min before the operation; donor rats in another group （Group HD-DA） were trea- ted by intraperitoneal injection of 5 - hydroxydeeanoate （5 mg / kg） 45 rain before the operation and intraperitoneal injection of diazoxide （5 mg / kg） 30 min before the operation; donor rats in the other group （Group C） underwent no drug pretreatment. Five recipients in each group were killed at 2 h, 1 d, 3 d, 5 d, and 7 d postoperatively. The levels of alanine aminotransferase （ALT） and aspartate aminotransferase（AST） were detected. Pathological examination was done by light microscope and elec- tro-microscope. Results： As compared with Group C, the liver cells of Group DA showed less swelling and necrosis; the struc ture of hepatic lobule of Group DA was less disorderly; and the inflammation in Group DA was less serious. The levels of ALT and AST at 2 h, l d, and 3 d post operatively in Group DA were significantly lower than those in Group C and Group HD-DA （P〈~0.05）. Conclusions： Diazoxide preconditioning have protective effect in partial liver transplantation in rats, but it can be specially blocked by 5 HD.

关 键 词：部分肝移植 二氮嗪 预处理 缺血再灌注损伤 大鼠 

分 类 号：R657.3[医药卫生—外科学]